Advertisement

Quantitative Ultrastructural Modifications in Human Spermatozoa after Freezing

  • Denise Escalier
  • Jean-Pierre Bisson

Abstract

Observations of decreased spermatozoa motility after freezing have motivated investigations attempting to identify ultrastructural cell damage from this procedure. As a result, lesions to the plasma membrane, acrosome and certain flagellar elements have been documented.1,7,10,12 However, these studies have been performed on spermatozoa frozen in cryoprotector media. Therefore, their findings are the result of a dual process: Cryo-aggression and cryo-protection. A better understanding of these two processes as well as the effect of the cryoprotector medium alone without freezing is needed and may lead to improved efficacy of semen cryopreservation. Of the studies that have approached the problem in this way,8,9 none have quantified their results.

Keywords

Seminal Plasma Human Spermatozoon External Membrane Fibrous Sheath Coarse Fiber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Behrman, S.J. Freeze preservation of human spermatozoa in liquid nitrogen vapor. Schweiz, Z. Gyn äk. Geburt sh; 2:307–313, 1971.Google Scholar
  2. 2.
    Bostrom, K. and Rubin, S.O. Destruction of spermatozoa in freezethawing of human semen. Scand. J. Urol. Nephrol., 7:120–122, 1973.PubMedCrossRefGoogle Scholar
  3. 3.
    David, G., Bisson, J.P., Czyglik, F., Jouannet P. and Gernigon, C. Que doit comporter 1’examen du sperme ? Comment en interpreter les résultats ? Feuillets de Biologie, XV:76, 1974.Google Scholar
  4. 4.
    David, G. and Czyglik, F. Tolerance à la congelation du sperme humain en fonction de la qualité initiale du sperme. J. Gyn. Obst. Biol. Repr., 6:601–610, 1977.Google Scholar
  5. 5.
    Drevius, L.O. and Eriksson, H. Osmotic swelling of mammalian spermatozoa. Experimental Cell Research, 42:136–156, 1966.PubMedCrossRefGoogle Scholar
  6. 6.
    Fawcett, D.W. The structure of the mammalian spermatozoon. Int. Rev. Ctyol., 7, 1958.Google Scholar
  7. 7.
    Friberg, J. and Nilsson, O. Motility and morphology of human sperms after freezing in liquid nitrogen. In: “Current Problems in Fertility”, Ingelman, Sundberg and Lunell, eds. Plenum Press, New-York-London, 1971.Google Scholar
  8. 8.
    Jones, R.C. Fertility and infertility in mammals in relation to sperm structure. In: The Biology of male gamete. J.G. Duckett and P.A. Racey, eds. Academic Press, London, pp. 343–365, 1975.Google Scholar
  9. 9.
    Matheson, G.W., Carlborg, L. and Gemzell, C. Frozen human semen for artificial insemination. Am. J. Obst. Gynec., 104:495–501, 1969.Google Scholar
  10. 10.
    Pedersen, H. and Lebech, P.E. Ultrastructural changes in the human spermatozoon after freezing for artificial insemination. Fertil. and Steril., 22:2, 1971.Google Scholar
  11. 11.
    Pedersen, H. The human spermatozoon. Dan. Med. Bull., .21 suppl. 1:3–36, 1974.Google Scholar
  12. 12.
    Schill, W.B. and Wolff, H. Ultrastructure of human sperm acrosome and determination of acrosin activity under conditions of semen preservation. Int. J. Fertil., 19:217–223, 1974.PubMedGoogle Scholar
  13. 13.
    Woolley, D.M. and Richardson, D.W. Ultrastructural injury to human spermatozoa after freezing and thawing. J. Repr od. Fert., 52:389–394, 1978.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Denise Escalier
    • 1
  • Jean-Pierre Bisson
    • 1
  1. 1.Centre d’Etude et de Conservation du Sperme at Paris-BicêtreKremlin-BicêtreFrance

Personalised recommendations