Neural Cell Lines
One promising approach to the study of the properties of nervous tissue is to use cells of neural origin that have adapted to permanent life in tissue culture. By obtaining cultures from individual cells, the resulting clonal cell lines should each comprise a homogeneous population, and the problems associated with the use of complex mixtures of cell types should be avoided. Since cloned cells divide in culture, large quantities of cells can be obtained for quantitative biochemical studies. The problem lies in finding cells that will grow readily in continuous culture yet still express specialized neural properties. Use of transformed cells is the rule, and hence the clonal cells will be genetically abnormal (but see Bulloch et al., 1977, for a different approach to establishing nerve cell lines).
KeywordsPC12 Cell Neuroblastoma Cell Muscarinic Receptor Mouse Neuroblastoma Mouse Neuroblastoma Cell
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