Diversity of Novel Proteins in Gonadal Fluids
Bovine seminal plasma contains four similar acidic proteins that we have previously designated as BSP-A1, BSP-A2, BSP-A3 and BSP-30kDa. These proteins, when added to pituitary cells grown in synthetic medium, result in an immediate release of gonadotropins in a manner similar to LH-RH. In lower concentrations they antagonize the action of LH-RH. BSP-A1, -A2 and -A3 have molecular weights between 15,000–16,000, while BSP-30kDa has a molecular weight of 30,000. BSP-A1, -A2 and -30kDa are glycoproteins containing neutral sugars, galactosamine and sialic acid. BSP-A1 and -A2 appear to be molecular variants that contain different amounts of carbohydrate but have an identical amino acid composition. BSP-A3 and -30kDa each has an amino acid composition different from that of -Al and -A2. BSP-A1/-A2 and -A3 each contain two similar domains that are arranged in tandem fashion and are homologous to the gelatin-binding domain of fibronectin as well as the amino-terminal end of human factor XII (blood clotting and fibrinolytic protease).
KeywordsSeminal Plasma Acrosome Reaction Luteinizing Hormone Release Hormone Denature Collagen Acquire Immuno Deficiency Syndrome
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