The Evolution of Adh Expression in the Repleta Group of Drosophila
The mechanisms by which gene expression is controlled in time, in a cell type specific manner and in response to physiologically active agents is currently under intense study using a variety of strategies. We have utilized an approach to this problem which offers several attractive features. This involves the identification of specific variation in a pattern of gene expression within a group of related species followed by identification of the DNA sequence elements which operate to control expression of the gene using functional tests. Subsequently, a comparison of the nucleotide sequence of these regions from each member of the species set should lead to hypotheses about specific nucleotides having a role in the control of at least one aspect of the gene’s pattern of expression. This hypothesis depends on the assumptions that sequence elements which are demonstrably conserved in different species are similar due to selection for a function, and that sequence differences may be regarded as candidates for the basis of the expression differences. Each of these can be tested by appropriate in vitro mutagenesis studies.
KeywordsTranscription Start Site Proximal Promoter Nurse Cell Distal Promoter Cell Type Specific Manner
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