Replication in Vitro of Adenovirus DNA

  • Peter C. van der Vliet
  • Bram G. M. van Bergen
  • Wim van Driel
  • Dick van Dam
  • Marijke M. Kwant
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)


The replication of the human adenovirus (Ad) DNA in infected cells is a highly efficient process. Within 40 hrs after infection a total amount of viral DNA is synthesized which almost equals the entire chromosomal DNA content of the cell. The abundance of replication proteins during infection has enabled the development of a replication system that initiates Ad DNA synthesis in vitro (1). So far, this is the only available system that replicates duplex DNA in higher eukaryotes. Since this system utilizes both viral and cellular proteins it may prove useful for the study of cellular DNA replication as well.


Nuclear Extract Inverted Terminal Repetition Initiation Reaction Terminal Protein Cytosol Factor 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Peter C. van der Vliet
    • 1
  • Bram G. M. van Bergen
    • 1
  • Wim van Driel
    • 1
  • Dick van Dam
    • 1
  • Marijke M. Kwant
    • 1
  1. 1.Laboratory for Physiological ChemistryState University of UtrechtUtrechtThe Netherlands

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