Analysis of Mu DNA Replicated on Cellophane Discs

  • N. Patrick Higgins
  • Purita Manlapaz-Ramos
  • Baldomero M. Olivera
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)

Abstract

The bacteriophage Mu is both a temperate bacterial virus and a transposable element. During the Mu lytic cycle, uncontrolled replicative transposition takes place. As a result, a single copy of Mu is amplified into ca. 100 copies each inserted at a different site in the host chromosome. During lytic infection or induction, when Mu replication is under viral control, replication is obligatorily coupled to transposition (1, 2).

Keywords

Recombination Triphosphate Alan Thymine dTTP 

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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • N. Patrick Higgins
    • 2
  • Purita Manlapaz-Ramos
    • 1
  • Baldomero M. Olivera
    • 1
  1. 1.Department of BiologyUniversity of UtahSalt Lake CityUSA
  2. 2.Department of BiochemistryUniversity of Alabama at BirminghamBirminghamUSA

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