Analysis of Mu DNA Replicated on Cellophane Discs
The bacteriophage Mu is both a temperate bacterial virus and a transposable element. During the Mu lytic cycle, uncontrolled replicative transposition takes place. As a result, a single copy of Mu is amplified into ca. 100 copies each inserted at a different site in the host chromosome. During lytic infection or induction, when Mu replication is under viral control, replication is obligatorily coupled to transposition (1, 2).
KeywordsRestriction Fragment Transposition Event Host Chromosome Lytic Infection Junction Fragment
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