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Replication of ΦX174 DNA by Calf Thymus DNA Polymerase-α: Measurement of Error Rates at the Amber-16 Codon

  • F. Grosse
  • G. Krauss
  • J. W. Knill-Jones
  • A. R. Fersht
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)

Abstract

It is now generally accepted that in higher eukaryotes DNA polymerase-α is the replicative enzyme (1). Recently we have purified to near homogeneity a subspecies of DNA polymerase-α (9S-enzyme), which contains a catalytically active core peptide (148 kd) and several smaller subunits (59 kd, 55 kd, and 48 kd). We have reported elsewhere (2) that the 9S polymerase-α is able to copy long stretches of single-stranded phage DNA. Furthermore, it contains a powerful primase activity. Thus, it can be regarded as a true replicative entity. In the present study we have replicated in vitro the single-stranded DNA of the bacteriophage ΦX174 (amber 16). The occurence of revertants to several temperature-sensitive mutants have been evaluated (3–5) in order to gain insight in the accuracy of the eukaryotic replicase.

Keywords

Mismatch Repair Reversion Frequency Replicative Enzyme Amber Codon Primase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • F. Grosse
    • 1
  • G. Krauss
    • 1
  • J. W. Knill-Jones
    • 2
  • A. R. Fersht
    • 2
  1. 1.Zentrum Biochemie, Abt. Biophysikalische ChemieMedizinische Hochschule HannoverHannoverGermany
  2. 2.Department of ChemistryImperial College of Science and TechnologyLondonUK

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