Replication of ΦX174 DNA by Calf Thymus DNA Polymerase-α: Measurement of Error Rates at the Amber-16 Codon

  • F. Grosse
  • G. Krauss
  • J. W. Knill-Jones
  • A. R. Fersht
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)


It is now generally accepted that in higher eukaryotes DNA polymerase-α is the replicative enzyme (1). Recently we have purified to near homogeneity a subspecies of DNA polymerase-α (9S-enzyme), which contains a catalytically active core peptide (148 kd) and several smaller subunits (59 kd, 55 kd, and 48 kd). We have reported elsewhere (2) that the 9S polymerase-α is able to copy long stretches of single-stranded phage DNA. Furthermore, it contains a powerful primase activity. Thus, it can be regarded as a true replicative entity. In the present study we have replicated in vitro the single-stranded DNA of the bacteriophage ΦX174 (amber 16). The occurence of revertants to several temperature-sensitive mutants have been evaluated (3–5) in order to gain insight in the accuracy of the eukaryotic replicase.


Mismatch Repair Reversion Frequency Replicative Enzyme Amber Codon Primase Activity 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • F. Grosse
    • 1
  • G. Krauss
    • 1
  • J. W. Knill-Jones
    • 2
  • A. R. Fersht
    • 2
  1. 1.Zentrum Biochemie, Abt. Biophysikalische ChemieMedizinische Hochschule HannoverHannoverGermany
  2. 2.Department of ChemistryImperial College of Science and TechnologyLondonUK

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