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Fidelity of DNA Replication in Vitro

  • Alan R. Fersht
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)

Abstract

DNA is replicated with a fidelity far higher than that expected from the specificity of Watson-Crick base pairing. The overall error rate in the replication of the chromosome of Escherichia coli is only one mistake in 108–1010 nucleotides polymerised (1) yet theoretical and experimental work suggests that non-complementary base pairing occurs at a frequency of up to 10-4 – 10-5 (2). There is strong evidence that the fidelity of DNA replication is boosted by editing or proofreading mechanisms that excise errors of misincorporation (3). For example, all known prokaryotic DNA polymerases have a 3′→5′-exo-nuclease activity that is specific for the removal of mismatched nucleotides from the growing 3′-terminus during replication (4). There are also post-replicative mismatch repair systems that patrol newly replicated DNA, repairing mismatches in the daughter strand (5).

Keywords

Exonuclease Activity Mismatched Nucleotide Fragment Primer Amber Mutant Daughter Strand 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Alan R. Fersht
    • 1
  1. 1.Department of ChemistryImperial College of Science and TechnologyLondonEngland

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