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A Ribonuclease H from Yeast Stimulates DNA Polymerase in Vitro

  • Robert Karwan
  • Hans Blutsch
  • Ulrike Wintersberger
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)

Abstract

Recently we have purified a ribonuclease-H (RNase-H) activity from the yeast, Saccharomyces cerevisiae (1) which differs in many of its properties (see Table 1) from ribonucleases-H described earlier for this organism by others (2, 3). The enzyme specifically degrades the RNA part of a DNA-RNA hybrid in an endonucleolytic mode and is completely inactive with single-stranded PNA or native DNA (Table 2).

Keywords

Glycerol Gradient Novikoff Hepatoma Standard Incubation Mixture Acid Soluble Nucleotide Glycerol Gradient Centrifuga 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Robert Karwan
    • 1
  • Hans Blutsch
    • 2
  • Ulrike Wintersberger
    • 1
  1. 1.Institute for Tumorbiology/Cancer ResearchUniversity of ViennaViennaAustria
  2. 2.Institute for MolecularbiologyUniversity of ViennaAustria

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