A Ribonuclease H from Yeast Stimulates DNA Polymerase in Vitro

  • Robert Karwan
  • Hans Blutsch
  • Ulrike Wintersberger
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)


Recently we have purified a ribonuclease-H (RNase-H) activity from the yeast, Saccharomyces cerevisiae (1) which differs in many of its properties (see Table 1) from ribonucleases-H described earlier for this organism by others (2, 3). The enzyme specifically degrades the RNA part of a DNA-RNA hybrid in an endonucleolytic mode and is completely inactive with single-stranded PNA or native DNA (Table 2).


Glycerol Gradient Novikoff Hepatoma Standard Incubation Mixture Acid Soluble Nucleotide Glycerol Gradient Centrifuga 
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  1. 1.
    Karwan, R., Blutsch, H. and Wintersberger, U. (1983) Biochemistry 22, 5500.CrossRefGoogle Scholar
  2. 2.
    Wyers, F., Sentenac, A. and Fromageot, P. (1976) Eur. J. Biochem. 69, 377.CrossRefGoogle Scholar
  3. 3.
    Wyers, F., Huet, J., Sentenac, A. and Fromageot, P. (1976) Eur. J. Biochem. 69, 385.CrossRefGoogle Scholar
  4. 4.
    Koerner, T.J. and Meyer, R.R. (1983) J. Biol. Chem. 258, 3126.Google Scholar
  5. 5.
    Crouch, R.J. and Dirksen, M.-L. (1982) in: “Nucleases” S.M. Linn and R.J. Roberts, eds., Cold Spring Harbor Laboratory, Cold Spring Harbor.Google Scholar
  6. 6.
    Dirksen, M.-L. and Crouch, R.J. (1981) J. Biol. Chem. 256, 11569.PubMedGoogle Scholar
  7. 7.
    Wintersberger, U. and Wintersberger, E. (1970) Eur. J. Biochem. 13, 11.PubMedCrossRefGoogle Scholar
  8. 8.
    Selzer, G. and Tomizawa, J.-I. (1982) Proc. Natl. Acad. Sci. USA 79, 7082.PubMedCrossRefGoogle Scholar
  9. 9.
    Hillenbrand, G. and Staudenbauer, W.L. (1982) Nucl. Acids Res. 10, 833.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Robert Karwan
    • 1
  • Hans Blutsch
    • 2
  • Ulrike Wintersberger
    • 1
  1. 1.Institute for Tumorbiology/Cancer ResearchUniversity of ViennaViennaAustria
  2. 2.Institute for MolecularbiologyUniversity of ViennaAustria

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