A Ribonuclease H from Yeast Stimulates DNA Polymerase in Vitro
Recently we have purified a ribonuclease-H (RNase-H) activity from the yeast, Saccharomyces cerevisiae (1) which differs in many of its properties (see Table 1) from ribonucleases-H described earlier for this organism by others (2, 3). The enzyme specifically degrades the RNA part of a DNA-RNA hybrid in an endonucleolytic mode and is completely inactive with single-stranded PNA or native DNA (Table 2).
KeywordsGlycerol Gradient Novikoff Hepatoma Standard Incubation Mixture Acid Soluble Nucleotide Glycerol Gradient Centrifuga
Unable to display preview. Download preview PDF.