In Vitro Replication of Rl Miniplasmid DNA

  • Ramon Diaz
  • Walter L. Staudenbauer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)


The antibiotic resistance plasmid Rl is composed of a cluster of drug resistance genes (r-determinant) and the resistance transfer factor (RTF). These two segments are flanked by directly repeated IS1 elements (ISla and ISlb). The RTF carries all the genetic information necessary for autonomous plasmid replication and conjugal transfer. The basic replicon of Rl (designated RepA) is situated adjacent to the ISlb element and includes the replication origin oriV and genes involved in replication and copy number control (see Figure 1). Rl replication is controlled by the 90 nucleotide copA RNA and the 11 kdal copB protein which act by inhibiting the expression of the 33 kdal repA protein (1). The repA protein is considered as the limiting factor for the initiation of Rl replication making plasmid DNA synthesis strictly dependent on de novo protein synthesis (2).


repA Protein Conjugal Transfer Plasmid Replication Drug Resistance Gene Oxolinic Acid 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Light, J. and Molin, S. (1983) EMBO J. 2, 93.PubMedGoogle Scholar
  2. 2.
    Uhlin, B.E. and Nordström, K. (1978) Mol. Gen. Genet. 165, 167.PubMedCrossRefGoogle Scholar
  3. 3.
    Molin, S. and Nordström, K. (1980) J. Bacteriol. 141, 111.PubMedGoogle Scholar
  4. 4.
    Diaz, R. and Staudenbauer, W.L. (1982) J. Bacteriol. 150, 1077.PubMedGoogle Scholar
  5. 5.
    Staudenbauer, W.L. (1976) Mol. Gen. Genet. 145, 273.PubMedCrossRefGoogle Scholar
  6. 6.
    Diaz, R., Nordstrom, K. and Staudenbauer, W.L. (1981) Nature 289, 326.PubMedCrossRefGoogle Scholar
  7. 7.
    Timmis, K.N., Danbara, H., Brady, G. and Lurz, R. (1981) Plasmid 5, 53.PubMedCrossRefGoogle Scholar
  8. 8.
    Oertel, W., Kollek, R., Beck, E. and Goebel, W. (1979) Mol. Gen. Genet. 171, 277.PubMedCrossRefGoogle Scholar
  9. 9.
    Orr, E. and Staudenbauer, W.L. (1981) Mol. Gen. Genet. 181, 52.PubMedCrossRefGoogle Scholar
  10. 10.
    Hillenbrand, G. and Staudenbauer, W.L. (1982) Nucl. Acids Res. 10, 833.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Ramon Diaz
    • 1
  • Walter L. Staudenbauer
    • 1
  1. 1.Abteilung SchusterMax-Planck-Institut für Molekulare GenetikBerlin 33Federal Republic of Germany

Personalised recommendations