Advertisement

Effect of UV Induced DNA Lesions on the Activity of Escherichia Coli DNA Topoisomerases: A Possible Role of these Enzymes in DNA Repair

  • Antonia M. Pedrini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)

Abstract

Modifications of DNA structure by a number of DNA damaging agents cause distortions resulting in the unwinding of the DNA helix. We have shown that the unwinding angle due to the formation of pyrimidine dimers in a circular DNA molecule is very small and inadequate to cause helix disruption. Although the alteration of DNA superhelicity produced by photodimers appeared very limited — at least 25 dimers were necessary in order to unwind a single topoisomer by one superhelical turn (1) — it was enough to affect the activity of Micrococcus luteus DNA topoisomerase I: one dimer per molecule inhibited the activity of this enzyme, by 5% (2).

Keywords

Micrococcus Luteus Post Replication Repair European Molecular Biology Organization Dependent Recovery Helix Disruption 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ciarrocchi, G. and Pedrini, A.M. (1982) J. Mol. Biol. 155, 177.PubMedCrossRefGoogle Scholar
  2. 2.
    Pedrini, A.M. and Ciarrocchi, G. (1983) Proc. Natl. Acad. Sci. USA 80, 1787.PubMedCrossRefGoogle Scholar
  3. 3.
    Ferro, A.M., Higgins, N.P. and Olivera, B.M. (1983) J. Biol. Chem. 258, 6000.PubMedGoogle Scholar
  4. 4.
    Wang, J.C. (1971) J. Mol. Biol. 55, 523.PubMedCrossRefGoogle Scholar
  5. 5.
    Pedrini, A.M. and Ciarrocchi, G. (1983) in: “Proceedings of the National Meeting of Italian Group of DNA repair”, Castellani, A., ed. (ENEA, Rome), p. 85.Google Scholar
  6. 6.
    Edenberg, H.J. (1983) Cold Spring Harbor Symp. Quant. Biol. 47, 379.PubMedCrossRefGoogle Scholar
  7. 7.
    Di Nardo, S., Voelkel, K.A., Sternglanz, R., Reynolds, A.E. and Wright, A. (1983) Cold Spring Harbor Symp. Quant. Biol. 47, 779.CrossRefGoogle Scholar
  8. 8.
    Pruss, G., Manes, S.H. and Drlica, K. (1982) Cell 31, 52.CrossRefGoogle Scholar
  9. 9.
    von Wright, A. and Bridges, B.A. (1981) J. Bacteriol. 146, 18.Google Scholar
  10. 10.
    Witkin, E.M. (1982) Biochimie 64, 549.PubMedCrossRefGoogle Scholar
  11. 11.
    Overbye, K.M., Basu, S.K. and Margolin, P. (1983) Cold Spring Harbor Symp. Quant. Biol. 47, 785.PubMedCrossRefGoogle Scholar
  12. 12.
    Cunningham, R.P., Wu, A.M., Shibata, T., Das Gupta, C. and Radding, C.M. (1981) Cell 24, 213.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Antonia M. Pedrini
    • 1
  1. 1.Istituto di Genetica Biochimica ed Evoluzionistica del C.N.R.PaviaItaly

Personalised recommendations