Effect of UV Induced DNA Lesions on the Activity of Escherichia Coli DNA Topoisomerases: A Possible Role of these Enzymes in DNA Repair

  • Antonia M. Pedrini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)


Modifications of DNA structure by a number of DNA damaging agents cause distortions resulting in the unwinding of the DNA helix. We have shown that the unwinding angle due to the formation of pyrimidine dimers in a circular DNA molecule is very small and inadequate to cause helix disruption. Although the alteration of DNA superhelicity produced by photodimers appeared very limited — at least 25 dimers were necessary in order to unwind a single topoisomer by one superhelical turn (1) — it was enough to affect the activity of Micrococcus luteus DNA topoisomerase I: one dimer per molecule inhibited the activity of this enzyme, by 5% (2).


Micrococcus Luteus Post Replication Repair European Molecular Biology Organization Dependent Recovery Helix Disruption 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Antonia M. Pedrini
    • 1
  1. 1.Istituto di Genetica Biochimica ed Evoluzionistica del C.N.R.PaviaItaly

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