Regenerating Rat Liver Topoisomerase II: Purification of the Enzyme and Catenation of DNA Rings
In search for the biological functions of these enzymes, several eukaryotic type II topoisomerases have been isolated from various organisms including Trypanosomatids (1), Yeast (2), Drosophila (3,4), Xenopus (5), Hela cells (6) and Rat liver (7). Comparison with their prokaryotic counterparts (8) suggested their possible involvement in several genetic processes such as DNA replication, recombination, transposition and repair. In addition, several lines of evidence indicated that topoisomerases are closely associated with chromatin (9, 10) and might play a role in the structural changes occuring within chromatin (condensation/decondensation).
KeywordsCatenation Reaction Catenation Factor Eukaryotic Type Hydroxylapatite Column Densitometric Profile
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