Regenerating Rat Liver Topoisomerase II: Purification of the Enzyme and Catenation of DNA Rings

  • Gilles Mirambeau
  • Catherine Lavenot
  • Michel Duguet
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)


In search for the biological functions of these enzymes, several eukaryotic type II topoisomerases have been isolated from various organisms including Trypanosomatids (1), Yeast (2), Drosophila (3,4), Xenopus (5), Hela cells (6) and Rat liver (7). Comparison with their prokaryotic counterparts (8) suggested their possible involvement in several genetic processes such as DNA replication, recombination, transposition and repair. In addition, several lines of evidence indicated that topoisomerases are closely associated with chromatin (9, 10) and might play a role in the structural changes occuring within chromatin (condensation/decondensation).


Catenation Reaction Catenation Factor Eukaryotic Type Hydroxylapatite Column Densitometric Profile 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Gilles Mirambeau
    • 1
  • Catherine Lavenot
    • 2
  • Michel Duguet
    • 1
  1. 1.Laboratoire d’Enzymologie des Acides NucleiquesUniversité Pierre et Marie CurieParisFrance
  2. 2.Institut de Recherches Scientifiques sur le CancerUnité d’EnzymologieVillejuifFrance

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