Structural and Functional Properties of DNA Polymerase α from Calf Thymus

  • Friedrich Grummt
  • Waltraud Albert
  • Gerd Zastrow
  • Andrea Schnabel
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)


DNA polymerase α, the enzyme most widely believed to catalyze chromosomal DNA replication in animal cells, is typically purified as a high molecular weight complex of multiple polypeptides, as revealed by SDS-polyacrylamide gel electrophoresis (1–11). The question of precise subunit structure of this enzyme and the structurefunction relationship of the individual polypeptides are subjects of our current research. We report here on the association of several functional properties -besides the catalytic DNA polymerizing activity- with the α-polymerase complex isolated from calf thymus, i.e. Ap4A binding, amino acyl tRNA synthetase, primase and 3′→5′ exonuclease- (proofreading-) activities.


Exonuclease Activity High Molecular Weight Complex Nondenaturing Condition Hydroxyapatite Column Tryptic Peptide Mapping 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Holmes, A.M., Hesslewood, I.P. and Johnston, I.R. (1976) Eur. J. Biochem. 62, 229.PubMedCrossRefGoogle Scholar
  2. 2.
    Hesslewood, I.P., Holmes, A.M., Wakeling, W.F. and Johnston, I.R. (1978) Eur. J. Biochem. 84, 123.PubMedCrossRefGoogle Scholar
  3. 3.
    Banks, G.R., Holloman, W.K., Kairis, M.V., Spanos, A. and Yarranton, G.T. (1976) Eur. J. Biochem. 62, 131.PubMedCrossRefGoogle Scholar
  4. 4.
    Fischer, P.A. and Korn, D. (1977) J. Biol. Chem. 252, 6528.Google Scholar
  5. 5.
    Banks, G.R., Boezi, J.A. and Lehman, I.R. (1979) J. Biol. Chem. 254, 9886.PubMedGoogle Scholar
  6. 6.
    Chen, Y.C., Bohn, E.W., Planck, S.R. and Wilson S.H. (1979) J. Biol. Chem. 254, 11678.PubMedGoogle Scholar
  7. 7.
    Grummt, F., Wâltl, G., Jantzen, A.-M., Hamprecht, K., Hübscher, U. and Kuenzle, C.C. (1979) Proc. Natl. Acad. Sci. USA 76, 6081.Google Scholar
  8. 8.
    McKunc, K. and Holmes, A.M. (1979) Nucleic Acids Res. 6, 3341.CrossRefGoogle Scholar
  9. 9.
    Méchali, M., Abadiedebat, J. and De Recondo, A.M. (1980) J. Biol. Chem. 255, 2114.PubMedGoogle Scholar
  10. 10.
    Villani, G., Sauer, B. and Lehman, I.R. (1980) J. Biol. Chem. 255, 9479.PubMedGoogle Scholar
  11. 11.
    Grosse, F. and Krauss, G. (1981) Biochem. 20, 5470.CrossRefGoogle Scholar
  12. 12.
    Albert, W., Grummt, F., Hübscher, U. and Wilson, S.H. (1982) Nucleic Acids Res. 10, 935.PubMedCrossRefGoogle Scholar
  13. 13.
    Hübscher, U., Spanos, A., Albert, W., Grummt, F. and Banks, G.R. (1981) Proc. Natl. Acad. Sci. USA 78, 6771.PubMedCrossRefGoogle Scholar
  14. 14.
    Rapaport, E., Zamecnik, P.C. and Baril, E.F. (1981) Proc. Natl. Acad. Sci. USA 78, 838.PubMedCrossRefGoogle Scholar
  15. 15.
    Castronejo, M., Fournier, M., Gatius, M., Gander, J.C., Labonesse, B. and Litvak, S. (1982) Biochem Biophys. Res. Commun. 107, 294.CrossRefGoogle Scholar
  16. 16.
    Helfman, W.B. (1973) Eur. J. Biochem. 32, 42.PubMedCrossRefGoogle Scholar
  17. 17.
    Crear, M. and Pearlman, R.E. (1974) J. Biol. Chem. 248, 3123.Google Scholar
  18. 18.
    McLennan, A.G. and Kier, H.M. (1975) Biochem. J. 151, 239.PubMedGoogle Scholar
  19. 19.
    Banks, G.R. and Yarranton, G.T. (1976) Eur. J. Biochem. 62, 143.PubMedCrossRefGoogle Scholar
  20. 20.
    Chang, L.M.S. (1977) J. Biol. Chem. 252, 1873.PubMedGoogle Scholar
  21. 21.
    Wintersberger, E. (1978) Eur. J. Biochem. 84, 167.PubMedCrossRefGoogle Scholar
  22. 22.
    Byrnes, J.J., Downey, K.M., Black, V.L. and So, A.G. (1976) Biochemistry 15, 2817.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Friedrich Grummt
    • 1
  • Waltraud Albert
    • 1
  • Gerd Zastrow
    • 1
  • Andrea Schnabel
    • 1
  1. 1.Institut für BiochemieUniversität WürzburgWürzburgGermany

Personalised recommendations