DNA Polymerase Activities of Mammalian Cell-Cycle Mutants and “Wild-Type” Cells in Different States of Proliferative Activity and Quiescence
Of the three major DNA-dependent DNA polymerases of animal cells, polymerase a usually exhibits high activities in proliferating cells, while activities are low during proliferative quiescence (1, 2). On the other hand, polymerases β and γ do not seem to be correlated with cell proliferation. The present communication is concerned with control of DNA polymerase activities in heat- and cold-sensitive cell-cycle mutants of a murine mastocytoma which enter qualitatively different states of reversible proliferative quiescence when incubated at the respective nonpermissive temperature (3, 4). In addition, DNA polymerase activities were studied in “wild-type” cells that are reversibly arrested in cell-cycle progression by butyrate or dimenthyl sulfoxide (DMSO), and in variants with relative resistance to these agents, thus permitting a comparison of DNA polymerase activities of homogeneous cell populations in exponential cell proliferation and in different states of proliferative quiescence.
KeywordsLabel Index Homogeneous Cell Population Cell Cycle Mutant Murine Mastocytoma Clonal Subline
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