The Origin of DNA Replication of Bacteriophage fl and its Interaction with the Phage Gene II Protein
The origin of DNA replication of bacteriophage fl consists of two functional domains: 1) a “core region”, about 40 nucleotides long, that is absolutely required for viral (plus) strand replication and contains three distinct but partially overlapping signals, a) the recognition sequence for the viral gene II protein, which is necessary for both initiation and termination of viral strand synthesis, b) the termination signal, which extends for 8 more nucleotides on the 5’ side of the gene II protein recognition sequence, c) the initiation signal that extends for about 10 more nucleotides on the 3’ side of the gene II protein recognition sequence; 2) a “secondary region”, 100 nucleotides long, required exclusively for plus strand initiation. Disruption of the “secondary region” does not completely abolish the functionality of the fl origin but does drastically reduce it (1% residual biological activity). This region, however, can be made entirely dispensable by mutations elsewhere in the phage genome.
KeywordsCold Spring Harbor Phage Genome Strand Synthesis Replicative Form Minus Strand
Unable to display preview. Download preview PDF.
- 1.Denhardt, D.T., Dressier, D. and Ray, D.S. eds. The Single-Stranded DNA Phages, (1978) Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
- 2.Kornberg, A. (1980) DNA replication. Freeman, San Francisco.Google Scholar
- 17.Dotto, G.P., Horiuchi, K. and Zinder, N.D. (1983) J. Mol. Biol. in press.Google Scholar