Inhibition and Recovery of the Replication of Depurinated Parvovirus DNA in Mouse Fibroblasts

  • J.-M. Vos
  • B. Avalosse
  • Z. Z. Su
  • J. Rommelaere
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)


Apurinic sites were introduced in the single-stranded DNA of parvovirus minute-virus-of-mice (MVM) and their effect on viral DNA synthesis was measured in mouse fibroblasts. Approximately one apurinic site per viral genome, is sufficient to block its replication in untreated cells. The exposure of host cells to a sublethal dose of UV-light 15 hours prior to virus infection, enhances their ability to support the replication of depurinated MVM. Cell pre-irradiation induces the apparent overcome of 10–15% of viral DNA replication blocks. These results indicate that apurinic sites prevent mammalian cells from replicating single-stranded DNA unless a recovery process is activated by cell UV-irradiation.


Apurinic Site Hairpin Region Strand Displacement Synthesis Terminal Palindrome 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • J.-M. Vos
    • 1
  • B. Avalosse
    • 1
  • Z. Z. Su
    • 1
  • J. Rommelaere
    • 1
  1. 1.Laboratory of Biophysics and RadiobiologyUniversité Libre de BruxellesRhode St. GenèseBelgium

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