Inhibition and Recovery of the Replication of Depurinated Parvovirus DNA in Mouse Fibroblasts
Apurinic sites were introduced in the single-stranded DNA of parvovirus minute-virus-of-mice (MVM) and their effect on viral DNA synthesis was measured in mouse fibroblasts. Approximately one apurinic site per viral genome, is sufficient to block its replication in untreated cells. The exposure of host cells to a sublethal dose of UV-light 15 hours prior to virus infection, enhances their ability to support the replication of depurinated MVM. Cell pre-irradiation induces the apparent overcome of 10–15% of viral DNA replication blocks. These results indicate that apurinic sites prevent mammalian cells from replicating single-stranded DNA unless a recovery process is activated by cell UV-irradiation.
KeywordsApurinic Site Hairpin Region Strand Displacement Synthesis Terminal Palindrome
Unable to display preview. Download preview PDF.
- 5.Defais, M.J., Hanawalt, P.C. and Sarasin, A.R. (1983) Adv. Radiat. Biol. 10, 1.Google Scholar
- 7.Ward, D.C. and Tattersall, P.J. (1982) in: “The Mouse in Biomedical Research”, Academic Press, New York.Google Scholar
- 8.Hardt, N., Dinsart, C, Spadari, S., Pedrali-Noy, G. and Rommelaere, J. (1983) J. gen. Virol. 64, in press.Google Scholar
- 9.Rose, J.A. (1975) Comprehensive Virology 3, 1.Google Scholar
- 10.Astell, C.R., Thomas, M., Chow, M.B. and Ward, D.C. (1982) Cold Spring Harbor Symp. Quant. Biol. 47, in press.Google Scholar
- 13.Teebor, G.W. and Brent, T.P. (1981) in: “DNA repair”, E.C. Friedberg and P.C. Hanawalt, eds., M. Dekker Inc., New York and Basel.Google Scholar