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Chromatin Structure and DNA Replication

  • Wolfgang Pülm
  • Rolf Knippers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 179)

Summary

The structure of (3H) thymidine pulse labeled, replicating chromatin differs from that of non replicating chromatin by several operational criteria which are related to the higher nuclease sensitivity of replicating chromatin. We summarize the structural changes that we observe using replicating chromatin as substrate for micro-coccal nuclease. The data suggest a more extended configuration of replicating compared to non replicating chromatin. We use these data to discuss a model of the chromatin structure in the vicinity of replication forks.

Finally, we present data to show that the reversion of structural changes in replicative chromatin depends on continued DNA replication.

Keywords

Chromatin Structure Sucrose Gradient Replication Fork Micrococcal Nuclease Sucrose Gradient Centrifugation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Wolfgang Pülm
    • 1
  • Rolf Knippers
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzGermany

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