Characterization of in Vitro DNA Synthesis in an Isolated Chloroplast System of Petunia Hybrida
The genetic organization and regulation of expression of chloroplast genes are extensively studied at this moment in order to answer questions about chloroplast biogenesis and the regulation thereof, a process in which both nuclear and chloroplast genes are involved (1, 2). However, until now only little attention has been paid to the process of the replication of the chloroplast genome and the regulation of this replication. To obtain more information about the proteins involved in these processes and their appearance during biogenesis, we started to analyze the DNA-synthesizing activity in isolated mature chloroplasts of Petunia hybrida. Not only because this plant is used by many research groups as a model plant for molecular and biological studies (3), but also because it has the advantage that several developmental processes in the biogenesis of chloroplasts can be induced in plant cell cultures (4, 5).
KeywordsSoluble Fraction Chloroplast Genome Chloroplast Gene Spinach Chloroplast Petunia Hybrida
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