Collagen Distribution in Endochondral Cartilage Obtained in Vitro from Dedifferentiated Cells
Cells obtained by enzymatic dissociation of stage 28–30 (1) chick embryo tibiae cultured adherent to plastic culture dishes, dedifferentiate and assume a fibroblast-like morphology. Dedifferentiated cells synthesize extracellular matrix proteins characteristic of committed prechondrogenic mesenchymal cells (i.e. type I collagen and fibronectin). Transfer of dedifferentiated cells to suspension culture induce their differentiation to single hypertrophic chondrocytes after a transient stage in which cells form aggregates.
KeywordsSuspension Culture Chick Embryo Cell Aggregate Hypertrophic Chondrocytes Matrix Vesicle
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- 3.C. Tacchetti, R. Quarto, L. Nitsch, D. J. Hartmann and R. Cancedda, In vitro morphogenesis of chick embryo endochondral cartilage, submitted for publication (1987).Google Scholar