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Collagen Distribution in Endochondral Cartilage Obtained in Vitro from Dedifferentiated Cells

  • C. Tacchetti
  • R. Quarto
  • R. Cancedda
Part of the Basic Life Sciences book series (BLSC, volume 48)

Abstract

Cells obtained by enzymatic dissociation of stage 28–30 (1) chick embryo tibiae cultured adherent to plastic culture dishes, dedifferentiate and assume a fibroblast-like morphology. Dedifferentiated cells synthesize extracellular matrix proteins characteristic of committed prechondrogenic mesenchymal cells (i.e. type I collagen and fibronectin). Transfer of dedifferentiated cells to suspension culture induce their differentiation to single hypertrophic chondrocytes after a transient stage in which cells form aggregates.

Keywords

Suspension Culture Chick Embryo Cell Aggregate Hypertrophic Chondrocytes Matrix Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    V. Hamburger and H. L. Hamilton, A series of normal stages in the development of the chick embryo, J. Morphol. 88:49 (1951).CrossRefGoogle Scholar
  2. 2.
    P. Castagnola, G. Moro, F. Descalzi-Cancedda and R. Cancedda, Type X collagen synthesis during in vitro development of chick embryo tibial chondrocytes, J. Cell. Biol. 102:2310 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    C. Tacchetti, R. Quarto, L. Nitsch, D. J. Hartmann and R. Cancedda, In vitro morphogenesis of chick embryo endochondral cartilage, submitted for publication (1987).Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • C. Tacchetti
    • 1
  • R. Quarto
    • 1
  • R. Cancedda
    • 1
  1. 1.Lab. of Cell DifferentiationNational Institute for Cancer ResearchGenoaItaly

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