Determination of Element Localization in Plant Tissue with the Microprobe

  • H. P. Rasmussen
  • V. E. Shull
  • H. T. Dryer
Part of the Developments in Applied Spectroscopy book series (DAIS, volume 6)


Elemental distribution of aluminum, calcium, phosphorus, and potassium in corn seedling root and shoot tissues was determined with the microprobe. The seedlings had been grown in quartz sand for two weeks and transferred to a nutrient culture containing 10-3 M AICI3 or CaCl2 for four days. The method of sample preparation was found to be critical to the localization of these elements. Aluminum and phosphorus were found in high concentrations at the root surface and within the root cap. Calcium and potassium, however, were distributed through the root and shoot tissues. Aluminum appeared to enter the plant conductive tissue through the break in the root surface caused by secondary root growth.


Root Surface Main Root Element Localize Corn Plant Secondary Root 
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Copyright information

© Chicago Section of the Society for Applied Spectroscopy 1968

Authors and Affiliations

  • H. P. Rasmussen
    • 1
  • V. E. Shull
    • 2
  • H. T. Dryer
    • 2
  1. 1.Michigan State UniversityEast LansingUSA
  2. 2.Applied Research LaboratoriesDearbornUSA

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