Application of Spectroscopy and Chromatography in Water Quality Analysis

  • W. DeWitt Johnson
  • Preston W. Kelly
Part of the Developments in Applied Spectroscopy book series (DAIS, volume 6)


This paper discusses spectroscopy and chromatography as they are being applied to obtain factual data and develop information for a comprehensive water-pollution control program. Spectroscopy and chromatography are employed in water-quality determinations to ascertain the levels of constituents such as nitrogens, phosphorus, gross minerals, organic content, and pesticides with the use of existing methods and modified or new methods developed in the Federal Water Pollution Control Administration’s Great Lakes Region-Chicago Program Office (GLR-CPO) laboratories. This paper also discusses the adaptation of automation in using an Auto- Analyzer for determining several water-quality parameters and touches upon future applications of spectroscopy and chromatography.


Methylene Blue Sulfanilic Acid Minimum Detectable Concentration Water Quality Analysis Federal Water Pollution Control 
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  1. 1.
    A.W. Breidenbach et al., The Identification and Measurement of Chlorinated Hydrocarbon Pesticides in Surface Waters, USDI, FWPCA, WP-22, Washington, D.C. (1966).Google Scholar
  2. 2.
    U.S. Dept. of Health, Education, and Welfare, Public Health Service Drinking Water Standard, PHS Pub. No. 956, Supt. of Documents, Washington, D.CAAA. (1962).Google Scholar
  3. 3.
    H.P. Buchfield, D.E. Johnson, and E.E. Storres, Guide to the Analysis of Pesticide Residues, Vols. I and II, U.S. Dept. of Health, Education, and Welfare, Supt. of Documents, I (l)-VIII, E.3.4 (1), Washington, D.CAAA. (1965).Google Scholar
  4. 4.
    Beckman, An Introduction to Spectrophotometry, Bull. 295B, Beckman Instruments, Inc., Fullerton, Calif. (1966).Google Scholar
  5. 5.
    R. Stock and C.B.F. Rice, Chromatographic Methods, Reinhold Publishing Corp., New York (1963), pp. 1–206.Google Scholar
  6. 6.
    J.L. Ternberg, M.D. and F.B. Hershey, M.D., Colorimetric Determination of Blood Ammonia, J. Lab. Clin. Med. 56:766–776 (1960).Google Scholar
  7. 7.
    J.E. O’Brien and J. Fiore, Ammonia, Nitrate, Nitrite, Chloride Determination by Automatic Analysis, Wastes Eng. (July 1962).Google Scholar
  8. N.S. Zaleiko, Simultaneous Application of Chemical Analysis and Physical Parameter histrumentation to Wastes, Instrument Society of America, 21st Annual ISA Conf. and Exhibit, New York, October 24–27, 1966 (preprint).Google Scholar
  9. 9.
    W.B. Sarles, “Algae and Metropolitan Wastes,” in Madison’s Lakes and Urbanization, U.S. Dept. Health, Education, and Welfare, Public Health Service, Robert A. Taft SEC, Cincinnati, Ohio (1960), pp. 10–22.Google Scholar
  10. 10.
    E.P. Gail, A.H. Molaf, and R.W. Shuman, Determination of Orthophosphate in Fresh and Saline Water, J. Am. Water Works Assoc. 57:917 (1965).Google Scholar
  11. 11.
    APHA, AWWA, and WPCF, Standard Methods for the Examination of Water and Wastewater, 12th ed.. New YorkAAA (1965).Google Scholar
  12. 12.
    P.W. Kelley and F.D. Fuller, “An Automated Method for Determining Magnesium in Water and Wastewater,” in Automation in Analytical Chemistry, Technicon Controls, Inc., Ardsley (Chauncey), New York (1966), pp. 266–269.Google Scholar
  13. 13.
    Water Pollution, Biology Field and Laboratory Manual, Great Lakes Region-Chicago Program Office, FWPCA, U.S. Dept. Health, Education, and Welfare, Chicago, lU. (1966).Google Scholar
  14. 14.
    V. H. Farmer, A Preliminary Study of Surface Currents along the Southern Shore of Lake Ontario, Unpublished internal report. Dept. Health, Education, and Welfare, U.S. Public Health Service, Great Lakes-Illinois River Basins Project, Chicago, 111. (1965).Google Scholar
  15. M.J. Fishman and S.C. Downs, Methods for Analysis of Selected Metals in Water by Atomic Absorption; Spectrographic Analysis of Natural Water, U.S. Geological Survey Water Supply Paper 1540-C, U.S. Govt. Printing Office, Washington, D.C., pp. 23–45.Google Scholar
  16. 16.
    Chemistry Laboratory Manual, Great Lakes Region-Chicago Program Office, U.S. Dept. I, FWPCA, Chicago, 111. (1967).Google Scholar

Copyright information

© Chicago Section of the Society for Applied Spectroscopy 1968

Authors and Affiliations

  • W. DeWitt Johnson
    • 1
    • 2
  • Preston W. Kelly
    • 1
    • 2
  1. 1.Organic Chemistry and General Chemistry LaboratoriesU. S. Department of the InteriorChicagoUSA
  2. 2.Federal Water Pollution Control AdministrationGreat Lakes Region — Chicago Program OfficeChicagoUSA

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