Gas Chromatographic Analysis of Alpha-Hydroxy Carboxylic Acids

  • Norman E. Hoffman
  • Peter J. Conigliaro
Part of the Developments in Applied Spectroscopy book series (DAIS, volume 4)


Dilute solutions of alpha-hydroxy acids in water can be analyzed by oxidation of the acids with periodic acid in the chromatograph. The quantity of aldehyde or ketone produced in oxidation is measured. When a Carbowax 20M-on-firebrick column is used, acid concentration is proportional to aldehyde or ketone peak height.


Peak Height Periodic Acid Methyl Ethyl Ketone Hydroxy Acid Mandelic Acid 


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Copyright information

© Chicago Section of the Society for Applied Spectroscopy 1965

Authors and Affiliations

  • Norman E. Hoffman
    • 1
  • Peter J. Conigliaro
    • 1
  1. 1.Marquette UniversityMilwaukeeUSA

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