Gas Chromatographic Analysis of Alpha-Hydroxy Carboxylic Acids

  • Norman E. Hoffman
  • Peter J. Conigliaro
Part of the Developments in Applied Spectroscopy book series (DAIS, volume 4)


Dilute solutions of alpha-hydroxy acids in water can be analyzed by oxidation of the acids with periodic acid in the chromatograph. The quantity of aldehyde or ketone produced in oxidation is measured. When a Carbowax 20M-on-firebrick column is used, acid concentration is proportional to aldehyde or ketone peak height.


Peak Height Periodic Acid Methyl Ethyl Ketone Hydroxy Acid Mandelic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    N. E. Hoffman, J.J. Barboriak, and H. F. Hardman, Analytical Biochemistry 9, 175 (1964).CrossRefGoogle Scholar
  2. 2.
    S. Siggia, Quantitative Organic Analysis via Functional Groups, John Wiley and Sons, Inc., New York (1949), p. 8.Google Scholar
  3. 3.
    P. Fleury and R. Boisson, J. Pharm. Chim. 30, 145 (1939).Google Scholar
  4. 4.
    P. Fleury and J. Courtois, Bull. soc. chim. France, 190 (1948).Google Scholar
  5. 5.
    D.B. Spoison and E. Chargoff, J. Biol. Chem. 164, 433 (1946).Google Scholar
  6. N. E. Hoffman, unpublished results.Google Scholar
  7. 7.
    W.G. Young, R. Dillon, and H.J. Lucas, J. Am. Chem. Soc. 51, 2528 (1929).CrossRefGoogle Scholar
  8. 8.
    P. A. Levenne and H. L. Haller, J. Biol. Chem. 77, 55 (1928).Google Scholar
  9. 9.
    W.M. Latimer and J.H. Hildebrand, Reference Book of Inorganic Chemistry, The Macmillan Co., New York (1940), p. 177.Google Scholar

Copyright information

© Chicago Section of the Society for Applied Spectroscopy 1965

Authors and Affiliations

  • Norman E. Hoffman
    • 1
  • Peter J. Conigliaro
    • 1
  1. 1.Marquette UniversityMilwaukeeUSA

Personalised recommendations