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Adaptation of an Inexpensive Ultraviolet-Visible Spectrophotometer for Enzyme Kinetic Work

  • David L. Heyse
Conference paper
Part of the Developments in Applied Spectroscopy book series (DAIS, volume 4)

Abstract

A Perkin-Elmer Model 202 spectrophotometer was modified to permit sensitive recording of absorbance changes with time by installing a retransmitting slidewire internally. The slidewire, which may be of a standard commercial type, was supplied with an exciting voltage from a battery-powered external circuit consisting of a fixed resistor and two ten-turn potentiometers to permit the use of a variable scale expansion. The output of the slidewire was connected directly to a self-balancing recording potentiometer of a type in common use. This adaptation makes possible an amplification of pen response by a factor of more than four without exceeding the capabilities of the spectrophotometer, It does not interfere with the normal function of the instrument in recording spectra. The modified instrument has been used in the kinetic analysis of several enzyme-catalyzed reactions.

Keywords

Enzyme Kinetic Absorbance Change Ascorbic Acid Oxidase Sensitive Recording Exciting Voltage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chicago Section of the Society for Applied Spectroscopy 1965

Authors and Affiliations

  • David L. Heyse
    • 1
  1. 1.Department of BiochemistryUniversity of ChicagoChicagoUSA

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