Programed-Temperature Preparative Chromatography
When we speak of preparative chromatographs we may mean either one or both of two distinctly different types of devices. The first type is a small production plant, a device intended to produce a large quantity of a known component of interest and generally of high value to the user. The second type is more closely related to analytical purposes. In this case, the user will place the sample into a preparative chromatograph for the primary purpose of obtaining a reasonably small but very useful pure fraction for further analysis by analytical chromatography; for use in a mass spectrometer, infrared spectrometer, or other analytical instrument; or for standard means of semimicroanalysis which are commonly available in organic and analytical laboratories. In designing a preparative chromatograph, an instrument company must first decide which type it is to be. If it is to be the first type, then such features as automatic sampling and automatic collection for repetitive handling on an unattended basis must be given primary consideration. On the other hand, if it is to be the second type of instrument, automation of the device is not necessary; since it is probable that the quantity of component which must be prepared will be small, the type of problem to which it will be applied will vary from day to day. The user who is interested in the device on this basis would consider it well worth his time and attention to watch the chromatograph while it is in operation. This fundamental decision will affect the scale and dimensions, and to a large extent the cost, of the equipment.
KeywordsFatty Acid Ester Theoretical Plate Radial Gradient Pure Fraction Model 222P
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