Muscarinic Cholinergic Receptor Localization by Radiohistochemistry

  • J. K. Wamsley
  • M. A. Zarbin
  • N. J. M. Birdsall
  • M. J. Kuhar
Part of the Advances in Behavioral Biology book series (ABBI, volume 25)


Following the development of binding methods for studying receptors in vitro (18), it was possible to develop radiohistochemical methods in parallel for the light microscopic localization of receptors (9,19). While one can examine the anatomical distribution of receptors by simple dissection and in vitro biochemical studies, the autoradiographic microscopic analyses provide several advantages over this latter approach. The two main advantages are that the histochemical procedures provide a great sensitivity in the measurement of receptors and an excellent level of anatomical resolution in their localization. Thus, these studies provide an accurate, quantitative, high resolution assessment of the distribution of receptors throughout the brain or other tissues. These investigations are a basis for understanding the widespread drug action in brain and elsewhere and are valuable complements to other histochemical methods for mapping functional neurotransmitter pathways.


Muscarinic Receptor Motion Sickness Vestibular Nucleus Nucleus Tractus Solitarii Muscarinic Cholinergic Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Birdsall, N.J.M., Burgen, A.S.V. and Hulme, E.C. (1978): Molec. Pharmacol. 14:723–736.Google Scholar
  2. 2.
    Birdsall, N.J.M. and Hulme, E.C. (1976): J. Neurochem. 24:7–16.CrossRefGoogle Scholar
  3. 3.
    Birdsall, N.J.M., Hulme, E.C. and Burgen, A.S.V. (1980): Proc. Rev. Soc. Lond. B 207:1–12.CrossRefGoogle Scholar
  4. 4.
    Hulme, E.C., Birdsall, N.J.M., Burgen, A.S.V. and Mehta, P. (1978): Molec. Pharmacol. 14:737–750.Google Scholar
  5. 5.
    Klemm, N., Murrin, L.C. and Kuhar, M.J. (1979): Brain Res. 169:1–9.CrossRefGoogle Scholar
  6. 6.
    Kuhar, M.J. (1976): IN Biology of Cholinergic Function (eds) A.M. Goldberg and I. Hanin, Raven Press, New York, pp. 3–28.Google Scholar
  7. 7.
    Kuhar, M.J. (1978): Ann. NY Acad. Sci. 311:35–48.CrossRefGoogle Scholar
  8. 8.
    Kuhar, M.J. (1978): Fed. Proc. 37:153–157.Google Scholar
  9. 9.
    Kuhar, M.J. (1978): IN Neurotransmitter Receptor Binding (eds) H.I. Yamamura, S.J. Enna and M.J. Kuhar, Raven Press, New York, pp. 113–126.Google Scholar
  10. 10.
    Kuhar, M.J. and Yamamura, H.I. (1974): Proc. Soc. Neurosci. 4:294.Google Scholar
  11. 11.
    Kuhar, M.J. and Yamamura, H.I. (1975): Nature 253:560–561.CrossRefGoogle Scholar
  12. 12.
    Kuhar, M.J. and Yamamura, H.I. (1976): Brain Res. 110:229–243.CrossRefGoogle Scholar
  13. 13.
    Murrin, L.C. and Kuhar, M.J. (1979): Brain Res. 177:279–285.CrossRefGoogle Scholar
  14. 14.
    Rotter, A., Birdsall, N.J.M., Burgen, A.S.V., Field, P.M., Hulme, E.C. and Raisman, G. (1979): Brain Res. Rev. 1:141–166.CrossRefGoogle Scholar
  15. 15.
    Rotter, A., Birdsall, N.J.M., Field, P.M. and Raisman, G. (1979) Brain Res. Rev. 1:167–184.CrossRefGoogle Scholar
  16. 16.
    Stumpf, W.E. and Roth, L.G. (1966): J. Histochem. Cytochem. 14:274–287.CrossRefGoogle Scholar
  17. 17.
    Wamsley, J.K., Zarbin, M., Birdsall, N. and Kuhar, M.J. (1980): Brain Res. (in press).Google Scholar
  18. 18.
    Yamamura, H.I., Enna, S.J. and Kuhar, M.J. (eds) (1978): Neurotransmitter Receptor Binding, Raven Press, New York.Google Scholar
  19. 19.
    Yamamura, H.I., Kuhar, M.J. and Snyder, S.H. (1974): Brain. Res. 810:170–176.CrossRefGoogle Scholar
  20. 20.
    Young, W.S. III and Kuhar, M.J. (1979): Brain Res. 179:255–270.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • J. K. Wamsley
    • 1
  • M. A. Zarbin
    • 1
  • N. J. M. Birdsall
    • 2
  • M. J. Kuhar
    • 1
  1. 1.Departments of Pharmacology and Experimental Therapeutics and Psychiatry and the Behavioral SciencesThe Johns Hopkins University School of Medicine BaltimoreMarylandUSA
  2. 2.National Institute for Medical ResearchMill Hill, LondonEngland

Personalised recommendations