Muscarinic Cholinergic Receptor Localization by Radiohistochemistry
Following the development of binding methods for studying receptors in vitro (18), it was possible to develop radiohistochemical methods in parallel for the light microscopic localization of receptors (9,19). While one can examine the anatomical distribution of receptors by simple dissection and in vitro biochemical studies, the autoradiographic microscopic analyses provide several advantages over this latter approach. The two main advantages are that the histochemical procedures provide a great sensitivity in the measurement of receptors and an excellent level of anatomical resolution in their localization. Thus, these studies provide an accurate, quantitative, high resolution assessment of the distribution of receptors throughout the brain or other tissues. These investigations are a basis for understanding the widespread drug action in brain and elsewhere and are valuable complements to other histochemical methods for mapping functional neurotransmitter pathways.
KeywordsMuscarinic Receptor Motion Sickness Vestibular Nucleus Nucleus Tractus Solitarii Muscarinic Cholinergic Receptor
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