Choline Transport and the Regulation of Acetylcholine Synthesis in Synaptosomes
In recent years evidence has accumulated supporting the proposal that the transport of choline (Ch) into nerve endings by the high affinity transport system may be the rate limiting step in acetylcholine (ACh) synthesis (for reviews see 6,9). We have investigated the interconnections between Ch transport and ACh synthesis with primary interest in: 1) the nature of the coupling between the high affinity transport of Ch and its acetylation catalyzed by choline acetyltransferase (ChAT), 2) in testing the possible existence of an intracellular pool of free Ch available for acetylation in nerve endings, and 3) in investigating the effects of varying extracellular concentrations of Ch on the synthesis and release of ACh.
KeywordsNerve Ending High Affinity Transport Acetylcholine Synthesis Striatal Synaptosome High Affinity Transport System
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