Choline Uptake and Choline Acetyltransferase in Brain of Developing Rats made Hypothyroid with Propylthiouracil
The development of the high affinity uptake of choline (Ch) in frontal cortex of rat brain, as measured using synaptosome (P2) fractions, was reported to be several days in advance of the development of choline acetyltransferase (ChAT, EC 220.127.116.11) activity, although adult values of both were evident by approximately 24 days of age (17). Of the 3H-Ch newly accumulated in small slices of frontal cortex by sodium dependent uptake, a larger fraction was acetylated in samples from adult rats than in samples from 7–14 day olds (2). The greater ChAT activity in brain of adult rats, which is accounted for largely by the basic molecular form of the enzyme, suggested that 3H-Ch is diluted into a pre-existing pool of Ch that is smaller in mature than in immature cortex. This interpretation was supported by an earlier observation of decreases during development in the concentration of Ch in rat brain, albeit of whole brain, concurrent with increases in ChAT activity (12).
KeywordsCholinergic Neurone Total Uptake Corpus Striatum ChAT Activity Striatal Slice
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