The Activity of Glycerophosphocholine Phosphodiesterase in Brain Tissue

  • G. B. Ansell
  • S. Spanner
Part of the Advances in Behavioral Biology book series (ABBI, volume 25)


Considerable interest has been generated in recent years about the supply of choline (Ch) for acetylcholine (ACh) synthesis in the brain (for short review see reference 4). This interest commenced with observations made over a decade ago that the capacity of brain tissue to synthesize Ch was non-existent, though this conclusion may have to be modified in view of more recent findings (23). Thus the brain has to assimilate Ch from the blood. There are some doubts whether the free Ch in the blood is the most immediate source of Ch for ACh synthesis and interest has therefore focussed on two problems. Is the Ch brought to the brain in a lipid-bound form and releases some Ch or is the lipid bound form assimilated into the pool of brain lipid -bound Ch from which Ch is released? The fact that there is in brain tissue a Ch-generating system (11,12,18), suggests that there is a mechanism for releasing Ch from Ch lipids in brain tissue which is enzymic in nature. The possible mechanisms have been discussed recently by Ansell and Spanner (4).


Brain Tissue Microsomal Fraction Synaptosomal Fraction Relative Specific Activity Somal Fraction 


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  1. 1.
    Abra, R.M. and Quinn, P.J. (1975): Biochim. Biophys, Acta 380:436–441.CrossRefGoogle Scholar
  2. 2.
    Ansell, G.B. and Chojnacki, T. (1963): IN Biochemical Problems of Lipids (ed) A.C. Frazer, Elsevier, Amsterdam, pp. 425–432.Google Scholar
  3. 3.
    Ansell, G.B. and Spanner, S. (1974): J. Neurochem. 22:1153–1155.CrossRefGoogle Scholar
  4. 4.
    Ansell, G.B. and Spanner, S. (1979): IN Nutrition and the Brain:Choline and Lecithin in Brain isorders, Vol. 5 (eds) A. Barbeau, J.H. Growdon and R.J. Wurtman, Raven Press, New York, pp. 35–46.Google Scholar
  5. 5.
    Baldwin, J.J. and Cornatzer, W.E. (1968): Biochim. Biophys. Acta 164:195–204.CrossRefGoogle Scholar
  6. 6.
    Baldwin, J.J., Lanes, P. and Cornatzer, W.E. (1969): Arch. Biochem. Biophys. 133:224–232.CrossRefGoogle Scholar
  7. 7.
    Dawson, R.M.C. (1956): Biochem. J. 62:689–693.Google Scholar
  8. 8.
    Diamond, I. and Milfay, D. (1972): J. Neurochem. 19:1899–1909.CrossRefGoogle Scholar
  9. 9.
    Domino, E.F., Schellenberger, M.K. and Frappier, J. (1968): Arch. Int. Pharmacodyn. Ther. 176:42–49.Google Scholar
  10. 10.
    Dross, K. (1975): J. Neurochem. 24:701–706.Google Scholar
  11. 11.
    Dross, K. and Kewitz, H. (1972): N.-S. Arch. Pharmacol. 274: 91–106.CrossRefGoogle Scholar
  12. 12.
    Freeman, J.J. and Jenden, D.J. (1976): Life Sci. 19:949–962.CrossRefGoogle Scholar
  13. 13.
    Gray, E.G. and Whittaker, V.P. (1962): J. Anat. (Lond) 96:79–88.Google Scholar
  14. 14.
    Illingworth, D.R. and Portman, O.W. (1973): Physiol. Chem. Phys. 5:365–373.Google Scholar
  15. 15.
    Jope, R.S. and Jenden, D.J. (1979): J. Neurosci. Res. 4:69–82.CrossRefGoogle Scholar
  16. 16.
    Lloyd-Davies, K.A., Michell, R.H. and Coleman, R. (1972): Biochem. J. 127:357–368.Google Scholar
  17. 17.
    Mann, S.P. (1975): Experientia 31:1256–1257.CrossRefGoogle Scholar
  18. 18.
    Schuberth, J., Sparf, B. and Sundwall, A. (1970): J. Neurochem. 17:461–468.CrossRefGoogle Scholar
  19. 19.
    Spanner, S. and Ansell, G.B. (1979): Biochem. J. 178:753–760.Google Scholar
  20. 20.
    Tattrie, N.H. and McArthur, C.S. (1958): Biochem. Preps. 6:16–19.Google Scholar
  21. 21.
    Webster, G.R., Marples, E.A. and Thompson, R.H.S. (1957): Biochem. J. 65:374–377.Google Scholar
  22. 22.
    Wolf, H.U. (1973): Experientia 29:241–249.CrossRefGoogle Scholar
  23. 23.
    Zeisel, S.H., Blusztajn, J.K. and Wurtman, R.J. (1979): IN Nutrition and the Brain: Choline and Lecithin in Brain Disorders, Vol. 5 (eds) A. Barbeau, J.H. Growdon and R.J. Wurtman, Raven Press, New York, pp. 47–55.Google Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • G. B. Ansell
    • 1
  • S. Spanner
    • 1
  1. 1.Department of PharmacologyUniversity of Birmingham Medical SchoolBirminghamEngland

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