Uptake of Acetylcholine into Torpedo Synaptic Vesicles InVitro
The discovery that brain synaptic vesicles contained acetylcholine (ACh)(6) seemed to confirm the earlier predictions of del Castillo and Katz (3) concerning the quantized nature of ACh released at the neuromuscular junction. Subsequently, with improvements in subcellular fractionation techniques, estimates for intravesicular ACh concentration have been continually revised upwards, and were recently reported to be as high as 0.8 M (8) in Torpedo vesicles.
KeywordsSynaptic Vesicle Vesicle Preparation Choline Acetyl Transferase Vesicular Uptake Porous Glass Bead
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