Isolation of Synaptic Vesicles from the Myenteric Plexus of Guinea Pig
The isolation of very pure cholinergic synaptic vesicles from the electromotor terminals of Torpedo (2,5) has provided much information which would also be useful to have for cholinergic synaptic vesicles from the mammalian peripheral nervous system. The only successful isolation of such vesicles is from ox superior cervical ganglia (7). We have now obtained a highly purified fraction of vesicles rich in acetylcholine (ACh) from the myenteric plexus-longitudinal muscle preparation (3) of guinea pig ileum.
KeywordsSynaptic Vesicle Myenteric Plexus Density Gradient Fractionation Zonal Rotor Soluble Cytoplasmic Protein
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