Electrophysiological and Morphological Correlates of the Re-Innervation of Rat Neuromuscular Junction: Implications on the Role of Membrane Components such as Gangliosides in the Motor Nerve Sprouting

  • A. Gorio
  • G. Carmignoto
  • M. Finesso
  • A. Leon
  • P. Marini
  • L. Tredese
  • R. Zanoni
Part of the Advances in Behavioral Biology book series (ABBI, volume 25)


The re-innervation process of a mammalian muscle can be divided into several parts: a) axonal growth; b) recognition of the endplate with consequent reformation of the nerve-muscle contact; c) maturation and stabilization of the new contact; and d) axonal sprouting, which can be considered a consequence of both the regeneration process and the presence of an endogenous stimulus, perhaps coming from the muscle. Our goal is to study those critical steps by means of histochemical and electrophysiological techniques. Three points mentioned above (b, c, d) were studied extensively, using frog neuromuscular preparations (6,7,18,24), and reviewed extensively by several authors (8,15). Although the studies performed on mammalian muscle are numerous, only a few approached the problem as a whole, trying to compare these different parameters (1) and carefully control the re-establishment of the synapse and the sprouting process itself.


Extensor Digitorum Longus Extensor Digitorum Longus Muscle Endplate Potential Induce Muscle Contraction Endplate Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bennett, M.R., McLachlan, E.M. and Taylor, R.S. (1973): J. Physiol. 233:481–500.Google Scholar
  2. 2.
    Bevan, S., Grampp, W. and Miledi, R. (1976):Proc. Roy. Soc. Lond. B 194:195–210.CrossRefGoogle Scholar
  3. 3.
    Carmignoto, G., Finesso, M., Tredese, L. and Gorio, A. (1981): IN Membranes, Molecules, Toxins and Cells, (eds) K. Block, L. Bolis and D.C. Tosteson, PSG Publications, Littleton (in press).Google Scholar
  4. 4.
    Ceccarelli, B., Aporti, F. and Finesso, M. (1976): Adv. Exp. Med. Biol. 71:275–293.Google Scholar
  5. 5.
    Clark, A.W., Mauro, A., Longenecker, H.E. Jr. and Hurlbut, W.P. (1970): Nature 225:703–705.CrossRefGoogle Scholar
  6. 6.
    Dennis, M.J. and Miledi, R. (1974): J. Physiol. 239:553–570.Google Scholar
  7. 7.
    Dennis, K.J. and Miledi, R. (1974): J. Physiol. 239:571–594.Google Scholar
  8. 8.
    Gorio, A. (1980): IN A Multidisciplinary Approach to Brain Development (ed) C. Di Benedetta, Elsevier North-Holland, Amsterdam, pp. 439–452.Google Scholar
  9. 9.
    Gorio, A., Carmignoto, G., Facci, L. and Finesso, M. (1980): Brain Res. 197:236–241.CrossRefGoogle Scholar
  10. 10.
    Gorio, A., Hurlbut, W.P. and Ceccarelli, B. (1978): J. Cell. Biol. 78:716–733.CrossRefGoogle Scholar
  11. 11.
    Gorio, A. and Mauro, A. (1979): Adv. Cyto pharmacol. 3:129–110.Google Scholar
  12. 12.
    Gorio, A. and Mauro, A. (1979): J. Gen. Physiol. 73:245–263.CrossRefGoogle Scholar
  13. 13.
    Gorio, A., Rubin, L.L. and Mauro, A. (1978): J. Neurocytol. 7:193–205.CrossRefGoogle Scholar
  14. 14.
    Gutmann, E. (1945): J. Anat. 79:1–8.Google Scholar
  15. 15.
    Harris, A.J. (1974): Ann. Rev. Physiol. 36:251–305.CrossRefGoogle Scholar
  16. 16.
    Hoffman, H. (1950): Aust. J. Exp. Biol. Med. Sci. 28:383–397.CrossRefGoogle Scholar
  17. 17.
    Hoffman, H. and Springeil, P.H. (1951): Aust. J. Exp. Biol. Med. Sci. 29:417–424.CrossRefGoogle Scholar
  18. 18.
    Letinsky, M.S., Fischbeck, K.H. and McMahan, U.J. (1976): J. Neurocytol. 5:691–718.CrossRefGoogle Scholar
  19. 19.
    Lomo, T. and Westgaard, R.H. (1975): J. Physiol. 252:603–626.Google Scholar
  20. 20.
    McArdle, J.J. and Albuquerque, E.X. (1973): J. Gen. Physiol. 61:1–23.Google Scholar
  21. 21.
    Miledi, R. (1960): J. Physiol. 154:190–205.Google Scholar
  22. 22.
    Obata, K., Oide, K. and Handa, S. (1977): Nature 266:369–371.CrossRefGoogle Scholar
  23. 23.
    Pestronk, A. and Drachman, D.B. (1978): Muscle & Nerve 1:70–74.CrossRefGoogle Scholar
  24. 24.
    Rotshenker, S. and McMahan, U.J. (1976): J. Neurocytol. 5:719–730.CrossRefGoogle Scholar
  25. 25.
    Tello, F. (1970): Trab. Lab. Invest. Biol. Univ. Madrid 5:117–151.Google Scholar
  26. 26.
    Yahara, I. and Kakirnoto-Sameshima, F. (1977): Proc. Nat. Acad. Sci. 74:4511–4515.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • A. Gorio
    • 1
  • G. Carmignoto
    • 1
  • M. Finesso
    • 1
  • A. Leon
    • 1
  • P. Marini
    • 1
  • L. Tredese
    • 1
  • R. Zanoni
    • 1
  1. 1.Fidia Research LaboratoriesAbano TermeItaly

Personalised recommendations