Advertisement

Acetylcholine Metabolism in PC12, A Clonal Cell Line on Secretory Cells

  • B. D. Howard
  • W. P. Melega
Part of the Advances in Behavioral Biology book series (ABBI, volume 25)

Abstract

PC12 is a clonal line of rat pheochromocytoma (7). PC12 cells synthesize catecholamines (primarily dopamine) and acetylcholine (ACh), store each in different granules and exhibit a Ca++-sensitive, depolarization-induced secretion of each (8,9,15,19). These properties make it possible to compare the metabolism and granular storage of two different transmitters produced by the same secretory cell. In this paper we summarize our use of PC12 cells for such studies, with the emphasis being on ACh.

Keywords

PC12 Cell Synaptic Vesicle Incubation Buffer ATPase Inhibitor Chromaffin Granule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bashford, C.L., Casey, R.P., Radda, G.K. and Ritchie, Q.A. (1976): Neuroscience 1:399–412.CrossRefGoogle Scholar
  2. 2.
    Breer, H., Morris, S.J. and Whittaker, V.P. (1977): Eur. J. Biochem. 80:313–318.CrossRefGoogle Scholar
  3. 3.
    Casey, R.P., Njus, D., Radda, G.K. and Sehr, P.A. (1977): Biochemistry 16:972–977.CrossRefGoogle Scholar
  4. 4.
    Collier, B. (1969): J. Physiol. 205:341–352.Google Scholar
  5. 5.
    Flatmark, T. and Ingebretsen, O.C. (1977): FEBS Lett. 78:53–56.CrossRefGoogle Scholar
  6. 6.
    Freeman, J.J., Choi, R.L. and Jenden, D.J. (1975): J. Neuro. chem. 24:729–734.Google Scholar
  7. 7.
    Greene, L.A. and Tischler, A.S. (1976): Proc. Natl. Acad. Sci. 73:2424–2428.CrossRefGoogle Scholar
  8. 8.
    Greene, L.A. and Rein, G. (1977): Brain Res. 129:247–263.CrossRefGoogle Scholar
  9. 9.
    Greene, L.A. and Rein, G. (1977): Nature 268:349–351.CrossRefGoogle Scholar
  10. 10.
    Johnson, R.G. and Scarpa, A. (1976): J. Biol. Chem. 251:2189–2191.Google Scholar
  11. 11.
    Johnson, R.G. and Scarpa, A. (1976): J. Gen. Physiol. 68:601–631.CrossRefGoogle Scholar
  12. 12.
    Johnson, R.G. and Scarpa, A. (1979): J. Biol. Chem. 254: 3750–3760.Google Scholar
  13. 13.
    Kuhar, M.J. and Murrin, L.C. (1978): J. Neurochem. 30:15–21.CrossRefGoogle Scholar
  14. 14.
    Mitchell, P. (1976): Biochem. Soc. Trans. 4:399–430.Google Scholar
  15. 15.
    Rebois, R.V., Reynolds, E.E., Toll, L. and Howard, B.D. (1980): Biochemistry 19:1240–1248.CrossRefGoogle Scholar
  16. 16.
    Richter, J.A. and Marchbanks, R.M. (1971); J. Neurochem. 18:691–703.CrossRefGoogle Scholar
  17. 17.
    Richter, J.A. and Marchbanks, R.M. (1973): J. Neurochem. 18:705–712.CrossRefGoogle Scholar
  18. 18.
    Rothlein, J.E. and Parsons, S.M. (1979): Biochem. Biophys. Res. Commun. 88:1069–1076.CrossRefGoogle Scholar
  19. 19.
    Schubert, D. and Klier, F.G. (1977): Proc. Natl. Acad. Sci. 74:5184–5188.CrossRefGoogle Scholar
  20. 20.
    Schubert, D., Heinemann, S. and Kidokoro, Y. (1977): Proc. Natl. Acad. Sci. 74:2579–2583.CrossRefGoogle Scholar
  21. 21.
    Schuldiner, S., Fishkes, H. and Kanner, B.I. (1978): Proc. Natl. Acad. Sci. 75:3713–3716.CrossRefGoogle Scholar
  22. 22.
    Toll, L. and Howard, B.D. (1978): Biochemistry 17:2517–2523.CrossRefGoogle Scholar
  23. 23.
    Toll, L. and Howard, B.D. (1980): J. Biol. Chem. 255:1787–1789.Google Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • B. D. Howard
    • 1
  • W. P. Melega
    • 2
  1. 1.Departments of Biological ChemistrySchool of Medicine, University of CaliforniaLos AngelesUSA
  2. 2.Departments of PharmacologySchool of Medicine, University of CaliforniaLos AngelesUSA

Personalised recommendations