Acetylcholine Biosynthesis in Developing Cholinergic Synapses

  • E. Giacobini
  • M. Marchi
Part of the Advances in Behavioral Biology book series (ABBI, volume 25)

Abstract

In contrast to in vitro conditions synapse formation in situ represents a long term process (16) consisting of consecutive steps of complex structural and biochemical modifications. On a chronological scale, synaptogenesis occurs not only during part of the embryonic phase but also during the period following birth or hatching. Recent studies have shown that in the avian peripheral nervous system the process of synaptic growth may continue throughout adult life (30). In the present study chick ciliary ganglia and their target organ (the iris) provides a useful model for investigating the process of synapse formation and growth (Fig. 1). For a comparison, the development of synapses in sympathetic ganglia (lumbar) was examined in a parallel study (14). The possibility of examining simultaneously cell bodies and terminals in the same group of cells belonging to a small (3000 cells) and well defined cholinergic population, represents a unique feature of this preparation (18). In this model, changes in biochemical parameters related to acetylcholine (ACh) metabolism can be correlated step by step to neurotransmission and morphology (15).

Keywords

Hydrolysis Migration Retina Trypsin Choline 

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References

  1. 1.
    Baughman, R.W. (1979): IN Developmental Neurobiology of Vision (ed) R.D. Freeman, Plenum Press, New York.Google Scholar
  2. 2.
    Betz, W. and Osborne, M. (1977): J. Physiol. 270:75–78.Google Scholar
  3. 3.
    Bunge, R., Johnson, M. and Ross, C.D. (1978): Science 199: 1409–1416.CrossRefGoogle Scholar
  4. 4.
    Burden, S. (1977): Develop. Biol. 57:317–329.CrossRefGoogle Scholar
  5. 5.
    Chiappinelli, V.A., Fairman, K. and Giacobini, E. (1978): Develop. Neurosci. 1:191–202.CrossRefGoogle Scholar
  6. 6.
    Chiappinelli, V. and Giacobini, E. (1978): Neurochem. Res. 3:465–478.CrossRefGoogle Scholar
  7. 7.
    Chiappinelli, V., Giacobini, E., Pilar, G. and Uchimura, H. (1976): J. Physiol. 257:749–766.Google Scholar
  8. 8.
    Cohen, A.M. (1972): J. Exp. Zool. 179:167–182.CrossRefGoogle Scholar
  9. 9.
    Coyle, J.T. and Yamamura, H.I. (1976): Brain Res. 118:429–449.CrossRefGoogle Scholar
  10. 10.
    Enna, S.J., Yamamura, H.J. and Snyder, S.H. (1976): Brain Res. 101:177–183.CrossRefGoogle Scholar
  11. 11.
    Fambrough, D. and Rash, J.E. (1971): Develop. Biol. 26:55–68.CrossRefGoogle Scholar
  12. 12.
    Fiori, M.G. and Mugnaini, E. (1979): Amer. Soe. Neurosci. Abst. 10:5.Google Scholar
  13. 13.
    Fisehbaeh, G.D. and Cohen, S.A. (1973): Develop. Bio. 31: 147–162.CrossRefGoogle Scholar
  14. 14.
    Giaeobini, E. (1978): IN Maturation of Neurotransmission (eds) E. Giaeobini, G. Filogamo and A. Vernadakis, S. Kargar AG, Basel, pp. 41–64.Google Scholar
  15. 15.
    Giaeobini, E. (1979): IN Neural Growth and Differentiation (eds) E. Meisami and M.A. Brazier, Raven Press, New York, pp.163–166.Google Scholar
  16. 16.
    Giacobini, E. (1980): IN Tissue Culture in Neurobiology (eds) E. Giaeobini, A. Shahar and A. Vernadakis, Raven Press, New Your, pp. 187–204.Google Scholar
  17. 17.
    Giacobini, E. (1980): IN Study Week in Neurobiology Nerve Cells, Transmitters and Behavior, Pontifical Academy, Rome, 45: 451–482.Google Scholar
  18. 18.
    Giacobini, E. and Chiappinelli, V. (1977): IN Synaptogenesis (ed) L. Tauc, Naturalia et Biologia Publications, Paris, pp. 89–116.Google Scholar
  19. 19.
    Giacobini, C., Filogamo, G., Weber, M., Boquet, P. and Changeux, J.P. (1973): Proc. Nat. Acad. Sci. USA 70.1708–1712.CrossRefGoogle Scholar
  20. 20.
    Giacobini, E., Pilar, G., Suszkiw, J. and Uchimura, H. (1979): J. Physiol. 286:233–253.Google Scholar
  21. 21.
    Inestrosa, N.C. and Fernandes, H.L. (1977): Neurosci. Lett.5: 91–93.CrossRefGoogle Scholar
  22. 22.
    Kouvelas, E.D. and Greene, L.A. (1976): Brain Res. 113:111–126.CrossRefGoogle Scholar
  23. 23.
    Kuhar, M.J. and Murrin, L.C. (1978): J. Neurochem. 30:15–21.CrossRefGoogle Scholar
  24. 24.
    Landmesser, L. and Pilar, G. (1972): J. Physiol. 222:691–713.Google Scholar
  25. 25.
    Landmesser, L. and Pilar, G. (1974): J. Physiol. 241:715–736.Google Scholar
  26. 26.
    Landmesser, L. and Pilar, G. (1974): J. Physiol. 241:737–749.Google Scholar
  27. 27.
    Landmesser, L. and Pilar, G. (1976): J. Cell Biol. 68:357–374.CrossRefGoogle Scholar
  28. 28.
    LeDouarin, N.M. (1977): IN Cell Interactions in Differentiation (ed) M. Karkinen-Jaaskelainen, Academic Press, London.Google Scholar
  29. 29.
    Le Douarin, N.M., Teillet, M.A., Ziller, C. and Smith, J. (1978): Proc. Nat. Acad. Sci. 75:2030–2034.CrossRefGoogle Scholar
  30. 30.
    Marchi, M. and Giacobini, E. (1980): Develop. Neurosci. 3:39–48.CrossRefGoogle Scholar
  31. 31.
    Marchi, M., Giacobini, E. and Hruschak, K. (1979): Develop; Neurosci. 2:201–212.CrossRefGoogle Scholar
  32. 32.
    Marchi, M., Hoffman, D.W., Giacobini, E. and Fredrickson, T. (1980): Develop. Neurosci. 3:235–247.CrossRefGoogle Scholar
  33. 33.
    Marchi, M., Hoffman, D.W., Giacobini, E. and Voile, R. (1980): Develop. Neurosci. (in press).Google Scholar
  34. Marchi, M., Hoffman, D.W., Mussini, I. and Giacobini, E. (1980) Develop. Neurosci. 3: 183–196.Google Scholar
  35. 35.
    Markow, R.M. (1979): Arkh. Anat. Histol. Embriol. (Russ.) 76.:21–27.Google Scholar
  36. 36.
    Mugnaini, E. (1975): Amer. Soc. Neurosci. Abst. 1146:744.Google Scholar
  37. 37.
    Patterson, P.H. (1978): Ann. Rev. Neurosci. 1:1–17.CrossRefGoogle Scholar
  38. 38.
    Patterson, P.H., Potter, D.D. and Furshpan, E.J. (1978): Sci. Amer. 239:50–59.CrossRefGoogle Scholar
  39. 39.
    Pilar, G., Jenden, D.J. and Campbell, B. (1973): Brain Res. 49:245–256.CrossRefGoogle Scholar
  40. 40.
    Rotundo, R., Chiappinelli, V.A., Giacobini, E. and Denenberg, V. (1977): Int. Soc. Neurochem. Satellite Symp. Abst.Google Scholar
  41. 41.
    Sastre, A., Gray, D.B. and Lane, A.M. (1977): Develop. Biol. 55:201–205.CrossRefGoogle Scholar
  42. 42.
    Scarsella, G., Toschi, G., Chiappinelli, V.A. and Giacobini, E. (1978): Develop. Neurosci. 16:133–141.CrossRefGoogle Scholar
  43. 43.
    Schuberth, J., Sparf, B. and Sundwall, A. (1969): J. Neurochem. 16.:605–700.Google Scholar
  44. Shainberg, A., Shahar, A., Burstein, M. and Giacobini, E. (1980): IN Tissue Culture in Neurobiology (eds) E. Giacobini, A. Shahar and A. Vernadakis, Raven Press, New York, pp.25–34.Google Scholar
  45. 45.
    Shelton, D.L., Nadler, J.M. and Cotman, C.W. (1979): Brain Res. 163:263–275.CrossRefGoogle Scholar
  46. 46.
    Smith, J., Fauquet, M., Ziller, C. and Le Douarin, N.M. (1979): Nature 282:853–855.CrossRefGoogle Scholar
  47. 47.
    Sorimachi, M. and Kataoka, K. (1975): Brain Res. 94:325–336.CrossRefGoogle Scholar
  48. 48.
    Sorimachi, M., Miyamoto, K. and Kataoka, K. (1974): Brain Res. 79:343–346.CrossRefGoogle Scholar
  49. 49.
    Vogel, Z. and Nirenberg, M. (1976); Proc. Nat. Acad. Sci. USA 73:1806–1810.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • E. Giacobini
    • 1
  • M. Marchi
    • 1
  1. 1.Laboratory of Neuropsychopharmacology, Department of Biobehavioral SciencesUniversity of Connecticut StorrsConnecticutUSA

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