Newly Developed Time-Resolved EPR Techniques for the Study of Cytochrome C Oxidase

  • Charles P. Scholes
  • Janet Bank
  • Chaoliang Fan
  • Harold Taylor


We present recent results from two new time-resolved EPR techniques used to probe structure and function of cytochrome c oxidase. The first of these, which we have used at cryogenic temperatures, is pulse field-sweep EPR. With this we can resolve hyperfine couplings of the CuA nucleus in cytochrome Oxidase, where such couplings are not directly resolved by straight EPR. This method is a technically simpler alternative to other double resonance techniques. The second technique is an ambient temperature technique to follow oxygen consumption by cytochrome oxidase. This technique uses an oxygen-sensitive spin probe to report the concentration of oxygen in solution. It is feasible to use this spin probe technique together with stopped flow. O2 kinetics have been followed both under aerobic conditions where O2 consumption is limited by cytochrome c reductant and under conditions where low oxygen concentration limits the rate of oxygen consumption.


Cytochrome Oxidase Hyperfine Coupling Spin Probe Microwave Pulse Field Sweep 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



(electron paramagnetic resonance)


(electron nuclear souble resonance)




(pulse field sweep EPR))


(continuous wave)



"spin amide"

(3-carbamoyl 2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy)


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Scholes, C.P., Janakiraman, R., Taylor, H., and King, T.E. (1984) Biophys. J. 45, 1027–1030CrossRefGoogle Scholar
  2. 2.
    Falkowski, K.M., Scholes, C.P., and Taylor, H. (1986) J. Magnetic Resonance 68, 453–468Google Scholar
  3. 3.
    Froncisz, W., Scholes, C.P., Hyde, J.S., Wei, Y-H., King, T.E., Shaw R.W., and Beinert, H., (1979) J. Biol. Chem. 254, 7482–7484Google Scholar
  4. 4.
    Lai, C-S, Hopwood, L.E., Hyde, J.S., and Lukiewicz, S. (1982) Proc. Nat’I. Acad. Sci. USA 79, 1166–1170CrossRefGoogle Scholar
  5. 5.
    Borg, D.C., (1964) Nature, 201, 1087–1090CrossRefGoogle Scholar
  6. 6.
    Froncisz, W., and Hyde, J.S. (1982) J. Magnetic Resonance 47, 515–521Google Scholar
  7. 7.
    Froncisz, W., Lai, C.S. and Hyde, J.S. (1985) Proc. Nat’I Acad. Sci. USA 82, 411–415CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Charles P. Scholes
    • 1
  • Janet Bank
    • 1
  • Chaoliang Fan
    • 1
  • Harold Taylor
    • 1
  1. 1.Department of PhysicsState University of New York at AlbanyAlbanyUSA

Personalised recommendations