Countercurrent Distribution Combined with Liquid Scintillation Counting of Double-Labeled Water-Soluble Radioactive Material in the Study of the Metabolism of Steroid Conjugates
Studies on the metabolism of conjugated forms of estriol [estra-1,3,5(10)-triene-3,16α,17β-triol] have been chosen to illustrate methods presently employed in our laboratory for the investigation of the metabolism of steroid conjugates. In previous experiments, it was found that, following the administration of labeled estriol to pregnant women volunteering for these studies, the radioactive material was recovered mainly, if not entirely, without any change of the steroid moiety . It was excreted in the form of various conjugates. Therefore, estriol appears to be an ideal compound for in vivo studies of steroid conjugation in the human being.
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