Countercurrent Distribution Combined with Liquid Scintillation Counting of Double-Labeled Water-Soluble Radioactive Material in the Study of the Metabolism of Steroid Conjugates

  • U. Goebelsmann
  • E. Diczfalusy


Studies on the metabolism of conjugated forms of estriol [estra-1,3,5(10)-triene-3,16α,17β-triol] have been chosen to illustrate methods presently employed in our laboratory for the investigation of the metabolism of steroid conjugates. In previous experiments, it was found that, following the administration of labeled estriol to pregnant women volunteering for these studies, the radioactive material was recovered mainly, if not entirely, without any change of the steroid moiety [1]. It was excreted in the form of various conjugates. Therefore, estriol appears to be an ideal compound for in vivo studies of steroid conjugation in the human being.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Wilson, R., Eriksson, G., and Diczfalusy, E., Acta Endocrinol. 46: 525 1964.PubMedPubMedCentralGoogle Scholar
  2. 2.
    Cohen, S.L., Marrian, G.F, and Odell, A.D., Biochem. J. 30: 2250 (1936).CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Menini, E., and Diczfalusy, E., Endrocrinology 68: 492 (1961).CrossRefGoogle Scholar
  4. 4.
    Beling, C.-G., Acta Endocrinol. Suppl. 79 (1963).Google Scholar
  5. 5.
    Diczfalusy, E., Barr, M., and Lind, J., Acta Endocrinol. 46: 511 (1964).PubMedGoogle Scholar
  6. 6.
    Cedard, L., and Diczfalusy, E. Quoted by Diczfalusy, E., in Martini, G. A. (editor), Aktuelle Probleme der Hepatologie, Georg Thieme, Stuttgart, 1962, p. 154.Google Scholar
  7. 7.
    Levitz, M., and Katz, J., Steroids 5: 11 (1965).CrossRefGoogle Scholar
  8. 8.
    Mikhail, G., Wiqvist, N., and Diczfalusy, E., Acta Endocrinol. 43: 213 (1963).PubMedGoogle Scholar
  9. 9.
    Bray, G.A., Anal. Biochem. 1: 279 (1960).CrossRefGoogle Scholar
  10. 10.
    Baggett, B., and Engel, L.L., J. Biol. Chem. 229: 443 (1957).Google Scholar
  11. 11.
    Goebelsmann, U., Sjóberg, K., Wiqvist, N., and Diczfalusy, E., Acta Endocrinol. 50: 261 (1965).Google Scholar
  12. 12.
    Goebelsmann, U., Eriksson, G., Wiqvist, N., and Diczfalusy, E., Acta Endocrinnol. 50: 273 (1965).Google Scholar

Copyright information

© New England Nuclear Corporation 1966

Authors and Affiliations

  • U. Goebelsmann
    • 1
  • E. Diczfalusy
    • 1
  1. 1.The Hormone Laboratory, Department of Women’s DiseasesKarolinska SjukhusetStockholmSweden

Personalised recommendations