Degradation of C14-Labeled Thymidine and its Halogenated Analogues in Man
The incorporation into DNA of mammalian cells in vitro of the halogenated thymidine analogues 5-bromo-, 5-iodo-, and 5-chlorodeoxyuridine (BUdR, IUdR, and CUdR) has been demonstrated in a number of laboratories, and many of the conditions promoting such incorporation have been defined. Such incorporation, when quantitatively adequate, renders the cells hypersensitive to X-irradiation . While these compounds may also be incorporated into DNA in vivo [2,3,4] quantitative substitution for DNA thymidine is greatly limited under these conditions because of the rapid phosphorolytic cleavage of the analogues [5,6]. To study the possibility of achieving a therapeutically useful degree of radiosensitization in man, it is important to be able to measure conveniently the amounts of the analogues incorporated under various conditions.
KeywordsLabel Compound Planimetric Measurement Tracer Methodology Activity aHer Ninth Symposium
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