Nucleic Acid Metabolism during Antibody Synthesis in Vitro

  • H. Kröger
  • H. Werchau
  • W. Hiby


Earlier experiments have shown that spleen cells isolated from rabbits immunized with alcohol dehydrogenase synthesize antibodies against the enzyme for as long as 50 hours [1,2]. We investigated the influence of various antimetabolites on this antibody synthesis. Among other results, we found a complete inhibition by puromycin and actinomycin D.


Biochemical Application Alcohol Dehydrogenase Spleen Cell Gradient Centrifugation Rabbit Serum 
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Copyright information

© New England Nuclear Corporation 1966

Authors and Affiliations

  • H. Kröger
    • 1
  • H. Werchau
    • 1
  • W. Hiby
    • 1
  1. 1.Department of Biochemistry Medical FacultyUniversity of FreiburgGermany

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