Synthesis and Metabolism of Glucose-3-H3 and Glucose-4-H3
It was established by Flatt and Ball  and in our laboratory [2,3] that TPNH generated in the pentose cycle is not sufficient to provide all the reducing equivalents for the biosynthesis of fatty acids in adipose tissue in vitro, when rates of synthesis are high. To extend these findings, we turned to the use of tritium-labeled substrates. Glucose-1-H3, glucose-6-H3, and H3 2O were used in mammary gland and adiposetissue [4, 5]. Results with these substrates were at variance with conclusions from previous work based on C14-labeled glucose. This was suspected to be the result of tritium isotope effects. It was deemed of value to prepare glucose labeled with tritium in positions 3 and 4. Glucose-3-H3 was previously used by Kemp and Rose.
KeywordsFatty Acid Synthesis Triose Phosphate Isomerase Tritiated Water Triose Phosphate Glyceric Acid
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