Electron Microscope Autoradiography
The opportunity to accurately localize radioactive substances in tissues at the cellular level became available with the introduction of relatively low energy beta-emitters, combined with the techniques of autoradiography. The major advantage offered by autoradiography is the ability to infer the location of individual molecules on the basis of the proximity of a reduced silver grain to this molecule. Therefore, attempts to increase the resolution of the method have utilized approaches that bring the two sites closer together. This paper will review some of the factors that relate to the theory and application of autoradiography when used with both light and electron microscopy.
Unable to display preview. Download preview PDF.
- 1.Berg, W.F., and Webb, J.H., in Mees, C.E.K. (editor), The Theory of the Photographic Process, Macmillan, New York, 1962, p. 147.Google Scholar
- 5.Bachmann, L., and Salpeter, M. M., in Bahr, G. F., and Zeitler, E.H. (editors), Quantitative Electron Microscopy, Williams and Wilkins, Baltimore, 1965, p. 303.Google Scholar
- 6.Pelc, S. R., J. Royal Microscop. Soc. 81: 131 (1963).Google Scholar
- 10.Caro, L.G., in Prescott, D.M. (editor), Methods in Cell Physiology, Vol. 1, Academic Press, New York, 1964, p. 327.Google Scholar
- 14.Ross, R., in Montagna, W., and Billingham, R.E. (editors), Advances in Biology of Skin: Wound Healing, Pergamon Press, London, 1964, p. 144.Google Scholar
- 22.Granboulan, P., J. Royal Microscop. Soc. 81: 165 (1963).Google Scholar
- 24.Ross, R., in Leblond, C. P., and Warren, K.B. (editors), The Use of Radio-autography in Investigating Protein Synthesis, Symposia of the International Society for Cell Biology, Vol. 4, Academic Press, New York, 1965, p. 273.Google Scholar