The In Vivo Use of Doubly-Labeled Glucose to Determine Cerebral Metabolism in Humans

  • William Sacks


An arterio-venous method was developed in this laboratory a number of years ago for the determination of cerebral metabolism in humans in vivo [1,2]. It consists briefly of giving the subject an intravenous injection of a labeled substrate and then drawing simultaneous arterial (femoral) and venous (internal-jugular bulb) blood samples at various time intervals. These blood samples are analyzed for radioactivity of the injected substrate, of the end product of metabolism of the substrate (carbon dioxide and/or water) and of intermediate metabolites (i.e., lactate and pyruvate). The increase in radioactivity of the end product in venous blood over its radioactivity in arterial blood is considered as due to catabolism of the substrate by the brain.


Citric Acid Cycle Cerebral Metabolism Tungstic Acid Krebs Cycle Intermediate Hexose Monophosphate Pathway 


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  1. 1.
    Sacks, W., J. Appl. Physiol. 9: 43 (1956).Google Scholar
  2. 2.
    Sacks, W., J. Appl. Physiol. 10: 37 (1957).Google Scholar
  3. 3.
    Sacks, W., J. Appl. Physiol. 14: 849 (1959).Google Scholar
  4. 4.
    Sacks, W., J. Appl. Physiol. 16: 1050 (1961).Google Scholar
  5. 5.
    Bloom, B., J. Biol. Chem. 234: 2158 (1959).Google Scholar
  6. 6.
    Calvin, M., Heidelberger, C., Reid, J. C., Tolbert, B. M., and Yankwich, P. E., “Isotopic Carbon,” Wiley, New York (1949).Google Scholar
  7. 7.
    Cohen, S. S., and Scott, D. B. M., Science 111: 543 (1950).CrossRefGoogle Scholar
  8. 8.
    Dent, C. E., Biochem. J. 41: 245 (1947).Google Scholar
  9. 9.
    Sunderman, F. W., Jr., and Sunderman, F. W., Am. J. Clin. Pathol. 36: 75 (1961).Google Scholar
  10. 10.
    Sacks, W., J. Appl. Physiol. 12: 311 (1958).Google Scholar
  11. 11.
    Berl, S., Takagaki, G., Clarke, D. D., and Waelsch, H., J. Biol. Chem. 237: 2562 (1962).Google Scholar
  12. 12.
    Margolis, R. V., Barkulis, S. S., and Geiger, A., J. Neurochem. 5: 379 (1960).CrossRefGoogle Scholar
  13. 13.
    Aprikyan, G. V., Doklady Akad. Nauk ArmSSR 35: 213 (1962).Google Scholar

Copyright information

© New England Nuclear Corporation 1965

Authors and Affiliations

  • William Sacks
    • 1
  1. 1.Research FacilityRockland State HospitalOrangeburgUSA

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