Advertisement

Liquid Scintillation Counting of C14-Labeled Amino Acids on Paper, Using Trinitrobenzene-1-Sulfonic Acid, and an Improved Combustion Apparatus

  • Claude F. Baxter
  • Ilse Senoner

Abstract

Methods for the separation of amino acids from tissue extracts by two-dimensional paper chromatography or by paper electrophoresis, have become well established in many laboratories. A number of one- and two-dimensional scanning devices have been developed for purposes of determining quantitatively the C14 radioactivity in amino acid spots on paper [1–4]. However the counting efficiency of these devices is low even when so-called 4π geometry is employed and both sides of the paper are counted simultaneously.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ludwig, H., Potter, V. R., Heidelberger, C., and Verdier, C. H., Biochim. Biophys. Acta 37: 525 (1960).CrossRefGoogle Scholar
  2. 2.
    Pocchiari, F., and Rossi, C., J. Chromatog. 5: 377 (1961).CrossRefGoogle Scholar
  3. 3.
    Perkins, H. J., and Tyrell, C., Can. J. Biochem. Physiol. 39: 1183 (1961).Google Scholar
  4. 4.
    Moses, V., and Lonberg-Holm, K. K., Anal. Biochem. 5: 11 (1963).CrossRefGoogle Scholar
  5. 5.
    Wang, C. H., and Jones, D. E., Biochem. Biophys. Research Commun. 1: 203 (1959).CrossRefGoogle Scholar
  6. 6.
    Lotfield, R. B., and Eigner, E. A., Biochem. Biophys. Research Commun. 3: 72 (1960).CrossRefGoogle Scholar
  7. 7.
    Takahashi, H., Hattori, T., and Maruo, B., Anal. Biochem. 2: 447 (1961).CrossRefGoogle Scholar
  8. 8.
    Kelly, R. G., Peets, E. A., Gordon, S., and Buyske, D. A., Anal. Biochem. 2: 267 (1961).CrossRefGoogle Scholar
  9. 9.
    Kàlberer, F., and Rutschmann, J., Heiv. Chim. Acta 44: 1956 (1961).CrossRefGoogle Scholar
  10. 10.
    Okuyama, T., and Satake, K., J. Biochem. (Tokyo) 47: 454 (1960).CrossRefGoogle Scholar
  11. 11.
    Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J. 38: 224 (1961).CrossRefGoogle Scholar
  12. 12.
    Dreyer, W. J., Unpublished data.Google Scholar
  13. 13.
    Baxter, C. F., Methods Med. Res. 9: 192 (1961).PubMedGoogle Scholar
  14. 14.
    Blair, A., and Segal, S., Anal. Biochem. 3: 221 (1962).CrossRefGoogle Scholar
  15. 15.
    Shinoda, T., and Satake, K., J. Biochem. (Tokyo) 50: 293 (1961).CrossRefGoogle Scholar
  16. 16.
    Katz, A.M., Dreyer, W. J., and Anfinsen, C.B., J. Biol. Chem. 324: 2897 (1959).Google Scholar

Copyright information

© New England Nuclear Corporation 1965

Authors and Affiliations

  • Claude F. Baxter
    • 1
  • Ilse Senoner
    • 1
  1. 1.Department of BiochemistryCity of Hope Medical CenterDuarteUSA

Personalised recommendations