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Liquid Scintillation Counting of C14-Labeled Amino Acids on Paper, Using Trinitrobenzene-1-Sulfonic Acid, and an Improved Combustion Apparatus

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Advances in Tracer Methodology

Abstract

Methods for the separation of amino acids from tissue extracts by two-dimensional paper chromatography or by paper electrophoresis, have become well established in many laboratories. A number of one- and two-dimensional scanning devices have been developed for purposes of determining quantitatively the C14 radioactivity in amino acid spots on paper [1–4]. However the counting efficiency of these devices is low even when so-called 4π geometry is employed and both sides of the paper are counted simultaneously.

This work was supported in part by grant NB-3743 from the National Institute of Neurological Diseases and Blindness, U.S. Public Health Service, Bethesda, Md. Presented at the Seventh Symposium on Advances in Tracer Methodology, March, 1963.

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© 1965 New England Nuclear Corporation

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Baxter, C.F., Senoner, I. (1965). Liquid Scintillation Counting of C14-Labeled Amino Acids on Paper, Using Trinitrobenzene-1-Sulfonic Acid, and an Improved Combustion Apparatus. In: Rothchild, S. (eds) Advances in Tracer Methodology. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8622-3_10

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  • DOI: https://doi.org/10.1007/978-1-4684-8622-3_10

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4684-8624-7

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