Liquid Scintillation Counting of C14-Labeled Amino Acids on Paper, Using Trinitrobenzene-1-Sulfonic Acid, and an Improved Combustion Apparatus
Methods for the separation of amino acids from tissue extracts by two-dimensional paper chromatography or by paper electrophoresis, have become well established in many laboratories. A number of one- and two-dimensional scanning devices have been developed for purposes of determining quantitatively the C14 radioactivity in amino acid spots on paper [1–4]. However the counting efficiency of these devices is low even when so-called 4π geometry is employed and both sides of the paper are counted simultaneously.
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