Some Experiences with Labeling Nucleotides

  • A. Markovitz
  • J. A. Cifonelli
  • J. I. Gross


Several years ago it became apparent that uridine nucleotides containing various carbohydrate moieties were the probable precursors of polysaccharides. This observation followed largely from the initial work of Leloir and his collaborators [1] and of Park [2]. Work by Cifonelli and Dorfman [3] showed that Group A streptococci contain UDP-glucuronic acid (UDPGA), UDP-N-acetylglucosamine (UDPAG), and UDP-N-acetylmuramic acid. The first two, UDPGA and UDPAG, contain the constituent monosaccharides of hyaluronic acid (HA), an acid mucopolysaccharide that occurs in connective tissues and in the capsule of certain Group A streptococci. Although reasonable quantities of the nucleotide precursors of HA were available, labeling with C14 would have been both difficult and time consuming.


Hyaluronic Acid Paper Chromatography Glucuronic Acid Specific Radioactivity Acid Mucopolysaccharide 
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Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • A. Markovitz
    • 1
  • J. A. Cifonelli
    • 1
  • J. I. Gross
    • 1
  1. 1.LaRabida-University of Chicago Institute and the Departments of Microbiology, Biochemistry, and PediatricsUniversity of ChicagoUSA

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