The Efficiency of Autoradiographic Stripping Film Applied to Tissue Sections Containing Tritiated Thymidine
Tritium-labeled thymidine has been extensively used in recent years to investigate the kinetics of cellular proliferation in a variety of normal and pathological tissues. It is known that thymidine is incorporated into the nuclei of cells synthesizing DNA prior to mitosis  but little quantitative information is available on its uptake by single cells. Also dependent upon quantitative data is the problem of radiation dosimetry, from intranuclear tritium, a problem with more than theoretical significance since it has been shown that tritiated thymidine can produce radiation damage and death in some of the labeled cells [2, 3]. For these purposes, measurements of total tritium activity in tissue samples  are inadequate, because of the nonuniform distribution of thymidine in living tissue and the short range of the tritium beta particle. Under these conditions, 90% of the energy of the disintegrations is dissipated within the nucleus  and the customary calculation of radiation dose in terms of energy absorbed per unit mass of tissue is inadequate. In order to correlate dose with biological effect the distribution of dose on a microscopic scale must be considered.
KeywordsLabel Cell Tritiated Thymidine Label Locus Autoradiographic Technique Counting Tube
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