The Efficiency of Autoradiographic Stripping Film Applied to Tissue Sections Containing Tritiated Thymidine

  • Walter E. Kisieleski
  • Renato Baserga
  • John Vaupotic


Tritium-labeled thymidine has been extensively used in recent years to investigate the kinetics of cellular proliferation in a variety of normal and pathological tissues. It is known that thymidine is incorporated into the nuclei of cells synthesizing DNA prior to mitosis [1] but little quantitative information is available on its uptake by single cells. Also dependent upon quantitative data is the problem of radiation dosimetry, from intranuclear tritium, a problem with more than theoretical significance since it has been shown that tritiated thymidine can produce radiation damage and death in some of the labeled cells [2, 3]. For these purposes, measurements of total tritium activity in tissue samples [4] are inadequate, because of the nonuniform distribution of thymidine in living tissue and the short range of the tritium beta particle. Under these conditions, 90% of the energy of the disintegrations is dissipated within the nucleus [5] and the customary calculation of radiation dose in terms of energy absorbed per unit mass of tissue is inadequate. In order to correlate dose with biological effect the distribution of dose on a microscopic scale must be considered.


Label Cell Tritiated Thymidine Label Locus Autoradiographic Technique Counting Tube 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. (1).
    Hughes, W. L., Bond, V. P., Brecher, G., Cronkite, E. P., Painter, R. B., Quastler, H., and Sherman, F.G. Proc. Natl. Acad. Sci. 44, 476–483 (1958).CrossRefGoogle Scholar
  2. (2).
    Johnson, H.A. and Cronkite, E. P. Radiation Research 11, 825–831 (1959).CrossRefGoogle Scholar
  3. (3).
    Lisco, H., Nishimura, E. T., Baserga, R., and Kisieleski, W. E. “The Effect of Tritiated Thymidine on the Growth of Ehrlich Ascites Tumor In Vivo.” Lab. Invest. 10, 435–443 (1961).Google Scholar
  4. (4).
    Baserga, R., Kisieleski, W. E., and Halvorsen, K. Cancer Research 20, 910–917 (1960).Google Scholar
  5. (5).
    Lajtha, L.G. and Oliver, R. Lab. Invest. 8, 214–221 (1959).Google Scholar
  6. (6).
    Herz, R. H. Nucleonics 9, 24–39 (1951).Google Scholar
  7. (7).
    Doniach, I. and Pelc, S. R. Brit. J. Radiol. 23, 184–192 (1950).CrossRefGoogle Scholar
  8. (8).
    Norris, W. P. and Woodruff, L.A. Ann. Rev. Nuclear Sci. 5, 297–326 (1955).CrossRefGoogle Scholar
  9. (9).
    Hughes, W. L. This volume, p. 291.Google Scholar
  10. (10).
    Cronkite, E. P. In: Stohiman, F. [ed.] The Kinetics of Cellular Proliferation, p. 137, Grune and Stratton, New York (1957).Google Scholar
  11. (11).
    Pelc, S.R. Intern. J. Appl. Radiation and Isotopes 1, 172–177 (1956).CrossRefGoogle Scholar
  12. (12).
    Lillie, R. D. “Histopathologic Technic,” The Blakiston Co., Philadelphia (1948).Google Scholar
  13. (13).
    Bernstein, W. and Ballentine, R. Rev. Sci. Instr. 21, 158 (1950).CrossRefGoogle Scholar
  14. (14).
    Wilzbach, K.E., Kaplan, L., and Brown, W.G. Science 118, 522 (1953).CrossRefGoogle Scholar
  15. (15).
    Pelc, S. R. Lab. Invest. 8, 127–129 (1959).Google Scholar
  16. (16).
    Messier, B. and Leblond, C. P. Proc. Soc. Exptl. Biol. Med. 96, 7–10 (1957).Google Scholar
  17. (17).
    Herz, R. H. Lab. Invest. 8, 71–75 (1959).Google Scholar
  18. (18).
    Tonna, E. A. and Cronkite, E. P. Stain Technol. 33, 255–260 (1958).Google Scholar
  19. (19).
    Schooley, J.C., Bryant, B. J., and Kelly, L. S. In: Stohlman, F. [ed.] “the Kinetics of Cellular Proliferation,” pp. 208–217, Grune and Stratton, New York (1957).Google Scholar
  20. (20).
    Robertson, J. S., Bond, V.P., and Cronkite, E. P. Intern. J. Appl. Radiation and Isotopes 7, 33–37 (1959).CrossRefGoogle Scholar

Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Walter E. Kisieleski
    • 1
    • 2
  • Renato Baserga
    • 1
    • 2
  • John Vaupotic
    • 1
    • 2
  1. 1.Division of Biological and Medical ResearchArgonne National LaboratoryUSA
  2. 2.Department of PathologyNorthwestern Medical SchoolUSA

Personalised recommendations