Autoradiography with Tritium

  • Walter L. Hughes


In autoradiography, a photographic emulsion is placed in contact with a specimen containing radioactivity and stored in the dark. The radiation, passing through the emulsion, sensitizes silver bromide granules so that they can be reduced to metallic silver by a photographic developer. One then sees a pattern of silver grains lying above the specimen. (The technique [1] employed in the present studies made use of stripping film which was floated over a histological preparation. In some cases Feulgen staining was performed before autoradiography; in other cases the specimen was stained through the emulsion after photographic development.)


Photographic Emulsion Tritiated Thymidine Label Thymidine Palladium Black Label Locus 
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Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Walter L. Hughes
    • 1
  1. 1.Brookhaven National LaboratoryUptonUSA

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