Abstract
In autoradiography, a photographic emulsion is placed in contact with a specimen containing radioactivity and stored in the dark. The radiation, passing through the emulsion, sensitizes silver bromide granules so that they can be reduced to metallic silver by a photographic developer. One then sees a pattern of silver grains lying above the specimen. (The technique [1] employed in the present studies made use of stripping film which was floated over a histological preparation. In some cases Feulgen staining was performed before autoradiography; in other cases the specimen was stained through the emulsion after photographic development.)
This research was supported by the Atomic Energy Commission. Presented at the First Symposium on Tritium in Tracer Applications, 1957.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Doniach and Pelc. “Autoradiograph Technique,” Brit. J. Radiol. 23, 184–192 (1950).
Robertson and Hughes. “Intranuclear Irradiation with Tritium-Labeled Thymidine,” Proc. 1st Natl. Biophysics Conf., p. 279, Yale Univ. Press (1959).
Fitzgerald, Eidinoff, Knoll, and Simmel. “Tritium in Radioautography,” Science 14, 494 (1951).
Kamen. “Isotopic Tracers in Biology,” Academic Press, New York (1957).
Taylor, Woods, and Hughes, Woods, and Hughes. “The Organization and Duplication of Chromosomes as Revealed by Autoradiographic Studies Using Tritium-Labeled Thymidine,” Proc. Natl. Sci. 43, 1, 122–28 (1957).
McQuade, Friedkin, and Atchison. “Radiation Effects of Thymidine 2-C14n Expt. Cell. Res. 11, 249–264 (1956).
Vendrely. In: Chargaff and Davidson [ed.], “The Nucleic Acids,” Vol. II, p. 155, Academic Press, New York (1955).
Hughes, Bond, Brecher, Cronkite, Painter, Quastler, and Sherman. “Cellular Proliferation in the Mouse as Revealed by Autoradiography with Tritiated Thymidine,” Proc. Natl. Sci. 44, 476 (1958).
Painter, Forro, and Hughes. “Distribution of Tritium-Labeled Thymidine in Escherichia cotiDuring Cell Multiplication,” Nature 181, 328–29 (1958).
Wilzbach. “Tritium Labeling by Exposure of Organic Compounds to Gas,” J. Am. Chem. Soc. 79, 1013 (1957).
Verly and Hunebelle have described a similar procedure. Bull. Soc. Chim. Belg. 66, 640 (1957).
Eidinoff and Knoll. “The Introduction of Isotopic Hydrogen into Purine Ring Systems by Catalytic Exchange,” J. Am. Chem. Soc. 75, 1992 (1953).
Pastore and Friedkin. “Transfer of Tritium from Tetrahydrofolate to the Methyl Group of Thymidylate,” J. Biol. Chem. (1962) (in press).
Hevesy and Ottesen. “Rate of Formation of Nucleic Acid in the Organs of the Rat,” Acta. Physiol. Scand. 5, 237–247 (1943).
Reichard and Estborn. “Utilization of Deoxyribosides in the Synthesis of Poly-nucleotides,” Biol. Chem. 188, 839–46 (1951).
Friedkin, Tilson, and Roberts, Tilson, and Roberts. “Studies of Deoxyribonucleic Acid Biosynthesis in Embryonic Tissues with Thymidine C14,” J. Biol. them. 220, 627 (1956).
Painter, Drew, and Hughes. “Inhibition of HeLa Growth by Intranuclear Tritium,” Science 127, 1244 (1958).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1963 New England Nuclear Corporation
About this chapter
Cite this chapter
Hughes, W.L. (1963). Autoradiography with Tritium. In: Rothchild, S. (eds) Advances in Tracer Methodology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8619-3_47
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8619-3_47
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8621-6
Online ISBN: 978-1-4684-8619-3
eBook Packages: Springer Book Archive