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Autoradiography with Tritium

  • Walter L. Hughes

Abstract

In autoradiography, a photographic emulsion is placed in contact with a specimen containing radioactivity and stored in the dark. The radiation, passing through the emulsion, sensitizes silver bromide granules so that they can be reduced to metallic silver by a photographic developer. One then sees a pattern of silver grains lying above the specimen. (The technique [1] employed in the present studies made use of stripping film which was floated over a histological preparation. In some cases Feulgen staining was performed before autoradiography; in other cases the specimen was stained through the emulsion after photographic development.)

Keywords

Photographic Emulsion Tritiated Thymidine Label Thymidine Palladium Black Label Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Walter L. Hughes
    • 1
  1. 1.Brookhaven National LaboratoryUptonUSA

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