Metabolism Studies by Radiorespirometry

  • Chih H. Wang


Many of the recent advances in the area of carbohydrate catabolism can be credited to the use of radiotracer methods. Although some tritium-labeled compounds have been used for these studies, the majority of research efforts in this regard involved the use of C14 specifically labeled carbohydrates or allied compounds. The catabolic mechanism for the utilization of a given carbohydrate is commonly elucidated by one or both of two basic approaches [1]. The first is the conversion of specifically labeled carbohydrate into catabolic products in the absence or presence of inhibitors. Isotopic distribution patterns and relative specific activity [2, 3] of a key product or an intermediate often provide important information on the nature and participation of an individual pathway. The discovery of the Entner-Doudoroff (ED) pathway [4] (Fig. 1) is the result of experiments of this type. The second approach is the examination of the respiratory C14O2 samples derived from specifically labeled carbon atoms of a given carbohydrate with respect to either the specific activities or yields. Experiments of this type are easy to carry out and provide information on the occurrence and participation of a known pathway in a given biological system.


Pulse Spectrum Liquid Scintillation Spectrometer Hexose Monophosphate Concurrent Operation Relative Specific Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Chih H. Wang
    • 1
  1. 1.Oregon State UniversityEugeneUSA

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