Advertisement

Tritium in Biochemical Studies

  • Maxwell L. Eidinoff

Abstract

When a portion of a biochemically interesting compound, such as methyl or phenyl, etc., remains intact throughout the experiment, then the hydrogens attached to the carbons may serve as a label for the carbons, or for the ring system. In this way, a purine or pyrimidine ring, a steroid framework, the phenyl group of an aromatic amino acid, a portion of fatty acid, has been used, when suitably labeled with tritium, as a marker for the group in question. This was one of the important uses for deuterium in biochemical systems, as shown in the pioneering work of Schoenheimer, Rittenberg, and numerous other investigators. In the text by Kamen, Radioactive Tracers in Biology, there is a chapter listing compounds containing deuterium and references are given to some applications with these compounds. It is, thus, simply necessary to state that those examples using stably bound deuterium carry over exactly to the use of tritium in those positions.

Keywords

Succinic Acid Aromatic Amino Acid Hydrogen Isotope Radioactive Tracer Pyrimidine Ring 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. (1).
    Farkas and Schneidmesser. J. Biol. Chem. 167, 807–809 (1947).Google Scholar
  2. (2).
    Thorn. Biochem. J. 49, 602 (1951).Google Scholar
  3. (3).
    Reitz and Bonhoeffer. Z. physik. Chem. (A) 172, 369 (1935).Google Scholar
  4. (4).
    Weinberger and Porter. Science 117, 636 (1953).CrossRefGoogle Scholar
  5. (5).
    Kamen. “Radioactive Tracers in Biology,” Chap. VII. Academic Press, New York (1951).Google Scholar
  6. (6).
    Schoenheimer. “Dynamic State of Body Constituents,” Harvard University Press, Cambridge, Mass. (1946).Google Scholar
  7. (7).
    Eidinoff, Perri, Knoll, Marano, and Arnheim. J. Am. Chem. Soc. 75, 248 (1953).CrossRefGoogle Scholar
  8. (8).
    Glascock and Dunscombe. “Communication to Proceedings of the Biochemical Society,” Biochem. J. 51, xl (1952).Google Scholar
  9. (9).
    Thompson and Ballou. Arch. Biochem. Biophys. 42, 219 (1953).CrossRefGoogle Scholar
  10. (10).
    Eidinoff, Knoll, Marano, Perri, and Rosenfeld. (Unpublished results.)Google Scholar
  11. (11).
    Verly, Rachele, du Vigneaud, Eidinoff, and Knoll. J. Am. Chem. Soc. 74, 5941 (1952).CrossRefGoogle Scholar
  12. (12).
    Du Vigneaud, Verly, Wilson, Rachele, Ressler, and Kinney. J. Am. Chem. Soc. 73, 2782 (1951).CrossRefGoogle Scholar

Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Maxwell L. Eidinoff
    • 1
  1. 1.Sloan-Kettering Institute for Cancer ResearchNew YorkUSA

Personalised recommendations