Experimental Design of Turnover Measurement Studies in the Unsteady State: Biosynthesis of Macromolecules

  • John M. Reiner

Abstract

Two questions that are often raised in connection with the biosynthesis of macromolecules, such as proteins, are:
  1. 1.

    When amino acid incorporation is measured in the absence of net synthesis, is this really measuring activity along a potential synthetic pathway, or an exchange such as might be produced by the action of a reversible hydrolytic enzyme?

     
  2. 2.

    Does biosynthesis of, e.g., proteins involve a template mechanism in which the entire molecule is assembled at a single site, or does it proceed by the stepwise building of ever larger intermediates?

     

Keywords

Hydrolysis Arthritis Polypeptide Macromolecule Triphosphate 

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References

  1. (1).
    Reiner, J. M. Arch. Biochem Biophys. 46, 53, 80 (1953).CrossRefGoogle Scholar
  2. (2).
    Jardetztry, C.D., and Barnum, C. P. Arch. Biochem. Biophys. 73, 435 (1958).CrossRefGoogle Scholar

Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • John M. Reiner
    • 1
  1. 1.Department of Microbiology, Division of Basic Health SciencesEmory UniversityUSA

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