Experimental Design of Turnover Measurement Studies in the Unsteady State: Biosynthesis of Macromolecules

  • John M. Reiner


Two questions that are often raised in connection with the biosynthesis of macromolecules, such as proteins, are:
  1. 1.

    When amino acid incorporation is measured in the absence of net synthesis, is this really measuring activity along a potential synthetic pathway, or an exchange such as might be produced by the action of a reversible hydrolytic enzyme?

  2. 2.

    Does biosynthesis of, e.g., proteins involve a template mechanism in which the entire molecule is assembled at a single site, or does it proceed by the stepwise building of ever larger intermediates?



Amino Acid Pool Label Amino Acid Entire Molecule Peptide Bond Formation Amino Acid Incorporation 
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  1. (1).
    Reiner, J. M. Arch. Biochem Biophys. 46, 53, 80 (1953).CrossRefGoogle Scholar
  2. (2).
    Jardetztry, C.D., and Barnum, C. P. Arch. Biochem. Biophys. 73, 435 (1958).CrossRefGoogle Scholar

Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • John M. Reiner
    • 1
  1. 1.Department of Microbiology, Division of Basic Health SciencesEmory UniversityUSA

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