Experimental Design of Turnover Measurement Studies in the Unsteady State: Biosynthesis of Macromolecules
When amino acid incorporation is measured in the absence of net synthesis, is this really measuring activity along a potential synthetic pathway, or an exchange such as might be produced by the action of a reversible hydrolytic enzyme?
Does biosynthesis of, e.g., proteins involve a template mechanism in which the entire molecule is assembled at a single site, or does it proceed by the stepwise building of ever larger intermediates?
KeywordsAmino Acid Pool Label Amino Acid Entire Molecule Peptide Bond Formation Amino Acid Incorporation
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