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Determination of Tritium and C14 in Biological Samples by Rapid Combustion Techniques

  • D. A. Buyske
  • R. Kelly
  • J. Florini
  • S. Gordon
  • E. Peets

Abstract

An important factor contributing to the increased use of tritium, and C14 as well, has been the availability of reliable and convenientto-operate commercial equipment that measures radioactivity by the liquid scintillation technique. This technique offers advantages of simplicity, sensitivity, and routine use that cannot be matched by alternative methods. However, inherent in liquid scintillation technique are two difficulties which make direct application to biological samples impractical. First, organic solvents are necessary for liquid scintillation counting. These are often immiscible with aqueous biological systems and are poor solvents for the polar constituents of biological samples. Second, liquid scintillation technique requires a relatively colorless sample so that the light resulting from the interaction of the beta particle with the phosphor in solution may reach the phototube. Any absorption of light manifests itself as a quenching, thus resulting in lowered counting efficiency.

Keywords

Spot Welding Combustion Method Nickel Foil Combustion Tube Liver Liver 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. (1).
    Kelly, R.G., Peets, E. A., Gordon, S., and Buyske, D.A. Anal. Biochem. 2, 267 (1961).CrossRefGoogle Scholar
  2. (2).
    Peets, E. A., Florini, J. R., and Buyske, D.A. Anal. Chem. 32, 1465 (1960).CrossRefGoogle Scholar
  3. (3).
    MacDonald, A.M. Analyst 86, 3 (1961).CrossRefGoogle Scholar
  4. (4).
    Davidson, J.D., and Feigelson, P. Int. J. Appl. Rad. and Isotopes 2, 1 (1957).CrossRefGoogle Scholar

Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • D. A. Buyske
    • 1
  • R. Kelly
    • 1
  • J. Florini
    • 1
  • S. Gordon
    • 1
  • E. Peets
    • 1
  1. 1.Lederle LaboratoriesPearl RiverUSA

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