Experiences with Tritiated Compounds Prepared by Exposure to Tritium Gas

  • Charles Rosenblum
  • Henry T. Meriwether


The Random Tritiation of organic compounds by contact with curie quantities of tritium gas [1] has greatly simplified the labeling of many organic compounds. Despite the absence of an adequate theory for predicting the specific activity attainable with a particular substance, the method has proven successful in labeling a great variety of compounds, including biologically active materials such as ribonucleic acid, lysozyme [2], and insulin [3]. In the Merck Sharp & Dohme Research Laboratories, this technique has been employed for the tritium labeling of a steroid, many substituted aromatic compounds, a partly saturated polycyclic structure, a poly-basic amino acid, a porphyrin-like structure, a sugar phosphate, and, in confirmation of an earlier report, [1] sucrose. Conditions prevailing during the tritiation of these compounds, as well as specific activities attained, are listed in Table I.


Gibberellic Acid Ultraviolet Absorption Sugar Phosphate Tritiated Water Tritium Atom 
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  1. (1).
    Wilzbach, K. E. J. Am. Chem. Soc. 79, 1013 (1957).CrossRefGoogle Scholar
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    Steinberg, D., Vaughan, M., Anfinsen, C. B., and Gorry, J. Science 126, 447 (1957).CrossRefGoogle Scholar
  3. (3).
    von Holt, C., and von Holt, L. Naturwiss. 12, 289 (1958).CrossRefGoogle Scholar

Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Charles Rosenblum
    • 1
  • Henry T. Meriwether
    • 1
  1. 1.Merck Sharp & Dohme Research LaboratoriesDivision of Merck & Co., Inc.RahwayUSA

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