Experiences with Tritiated Compounds Prepared by Exposure to Tritium Gas
The Random Tritiation of organic compounds by contact with curie quantities of tritium gas  has greatly simplified the labeling of many organic compounds. Despite the absence of an adequate theory for predicting the specific activity attainable with a particular substance, the method has proven successful in labeling a great variety of compounds, including biologically active materials such as ribonucleic acid, lysozyme , and insulin . In the Merck Sharp & Dohme Research Laboratories, this technique has been employed for the tritium labeling of a steroid, many substituted aromatic compounds, a partly saturated polycyclic structure, a poly-basic amino acid, a porphyrin-like structure, a sugar phosphate, and, in confirmation of an earlier report,  sucrose. Conditions prevailing during the tritiation of these compounds, as well as specific activities attained, are listed in Table I.
KeywordsGibberellic Acid Ultraviolet Absorption Sugar Phosphate Tritiated Water Tritium Atom
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