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Monitoring Gas Chromatography for H3- and C14-Labeled Compounds by Liquid Scintillation Counting

  • Herbert J. Dutton

Abstract

Isotopic assay of the isolated products from gas chromatography has frequently posed a difficult problem because of the small amounts of individual components isolated. When starting materials or reaction products are of relatively high specific activity (e.g., 0.1 mc/mg and higher), ion-chamber procedures of monitoring in gas chromatography are readily and effectively carried out [1], even at high temperatures [2]. This high-temperature technique, used for studying reaction products from the tritium-exposure procedures [3–7] and for C14-labeled compounds of high specific activity, has been reported [2]. Proportional counting has been used directly on effluents from gas chromatographic columns [8] and after combustion of all eluted material to carbon dioxide over heated copper oxide [9]. Differential-type curves are obtained by ratemeter-recorder systems. Unfortunately, much biological and organic research is conveniently or necessarily carried out at low levels of specific activity. For example, if 4 mc of C14-labeled linoleic acid is administered in human experimentation, many orders of dilution will be experienced if complete equilibration of the C14-labeled linoleic acid occurs in body linoleic acid; indeed, the amount of radioactivity subsequently isolated from gas chromatography becomes very small. A system of mixing a scintillation solution with the exit gas stream from a chromatographic column and circulating the solution over the face of a photomultiplier tube has been described by Popják et al. [10]. In this way they obtained an integral curve for radioactivity concurrently with a differential “analytical” curve from their gas density balance.

Keywords

Liquid Scintillation Methyl Oleate Methyl Linolenate High Specific Activity Linolenate Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Herbert J. Dutton
    • 1
  1. 1.Northern Utilization Research and Development DivisionDepartment of AgriculturePeoriaUSA

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